CELL-CELL INTERACTION BETWEEN ORAL ACTINOMYCETES AND OTHER ORAL BACTERIA

口腔放线菌和其他口腔细菌之间的细胞间相互作用

• 批准号: 3753502
• 负责人: P E KOLENBRANDER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3753502
• 关键词: Actinomycetales; Bacteroides gingivalis; adhesin; bacterial genetics; cell cell interaction; ecology; genetic strain; molecular cloning; molecular pathology; mutant; nucleic acid probes; nucleic acid sequence; open reading frames; oral bacteria; receptor binding; tooth surface; transposon /insertion element

The focus of our research program is to understand the role of coaggregation in bacterial accretion of early colonizing bacteria on a clean tooth surface. The primary colonizers include actinomyces and streptococci. Antiserum against the 34.8-kDa surface-adhesin, ScaA, from Streptococcus gordonii PK488 cross reacts with a similar size protein from all 14 streptococcal strains so far tested that coaggregate with Actinomyces naeslundii PK606. The sequence of the 6.125 kb clone that contains scaA revealed six open reading frames. The adhesin gene is located adjacent to two genes encoding an ATP-binding protein and a hydrophobic membrane protein. These three genes are homologous to a cassette of genes encoding binding-protein dependent transport systems in several other bacteria. ScaA is a lipoprotein which probably is anchored in the cell membrane while its adhesin binding site is exposed to the environment. Two other oral streptococci also have the same genetic organization of these genes, suggesting that the adhesin is a critical function for colonization of oral streptococci. A 100-kDa protein thought to mediate intrageneric coaggregation among streptococci is absent in several spontaneous mutants and one transposon-inactivated mutant of Streptococcus gordonii DL1. Two potential coaggregation-mediating adhesins from Actinomyces serovar WVA963 strain PK1259 have been identified. Binding of oral actinomyces to defined glycolipid molecules appears to follow the sema specificity exhibited by the actinomyces coaggregations with oral streptococci. The long range goal of these studies, collectively, is to elucidate the molecular mechanisms responsible for bacterial colonization in the human oral ecosystem.

我们研究计划的重点是了解 早期定殖细菌的共聚集 清洁牙齿表面。 主要的殖民者包括 放线菌和链球菌。 针对34.8-kDa的抗血清 格氏链球菌PK 488表面粘附素ScaA 与来自所有14种链球菌的相似大小的蛋白质反应 迄今为止测试的与内氏放线菌共聚集的菌株 PK606. 含有scaA的6.125 kb克隆的序列 显示了六个开放的阅读框架。 粘附素基因位于 与两个编码ATP结合蛋白的基因相邻， 疏水膜蛋白 这三个基因与 编码结合蛋白依赖性转运的基因盒 其他几种细菌的系统。 ScaA是一种脂蛋白， 当其粘附素结合时，可能锚定在细胞膜上 场地暴露于环境中。 其他两种口腔链球菌 也有相同的遗传组织的这些基因，这表明 粘附素是口腔黏膜上皮细胞定植的关键功能， 链球菌。 一种被认为介导属内共聚集的100 kDa蛋白质 在几种自发突变体和一种 戈登链球菌DL 1的转座子失活突变体。 两 来自血清型放线菌的潜在聚集介导粘附素 已鉴定出WVA 963菌株PK 1259。 口头约束力 放线菌定义的糖脂分子似乎遵循 放线菌共聚集体的sema特异性 口腔链球菌 这些研究的长期目标是， 共同的目的是阐明 人类口腔生态系统中的细菌定植。