EVALUATIONS OF GENETIC TOXICITY TEST RESULTS

遗传毒性试验结果评价

• 批准号: 3855831
• 负责人: E ZEIGER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3855831
• 关键词: Salmonella; carcinogen testing; chemical carcinogen; chemical carcinogenesis; chemical information system; chemical structure function; computers; mutagen testing; mutagens; statistics /biometry; toxicant screening

A database of results has been created using chemicals tested by NTP for mutagenesis in short-term assays, and for carcinogenesis. This database allows the evaluation of each short-term assay with respect to its ability to predict carcinogenesis or other short-term assay results. It also permits studies of the individual assays with respect to inter- and Intra-laboratory reproducibility. A study to examine the predictivity of the four short-term tests as a function of the potency of the short-term test and carcinogenicity test responses is continuing. The chemicals to be evaluated include the 114-chemical dataset used , previously to correlate qualitative short-term test results with carcinogenicity results, which is being used to correlate the same parameters using the potencies of the test responses. These measures of potency are being developed and evaluated, and will be used for the correlational studies. The predictivities for Salmonella mutagenicity of two computer-based structure-activity systems, one empirical structure-activity system, and one physicochemical system, were determined. The test database contained 100 chemicals selected at random from among chemicals that had teen tested for mutagenicity in Salmonella, but whose results had not been published by the NTP. The chemical structures were drawn and sent, without identifying them by name, or coded chemicals, to the participants. The purpose of the study was threefold: to measure the accuracy of the different test systems as they were configured at the time of the study: to use the results of this exercise to identify the strengths and weaknesses' of each system: and to bring understanding and light to structure-activity predictions. The two computer-based SAR systems and the empirical system gave equivalent predictivities (approx. 70-80%), while the physicochemical system was not as effective (approx. 60% predictivity). The analyses of the results, and a determination of the differences and similarities among the various systems is continuing.

已使用NTP测试的化学品创建了结果数据库 短期试验中的诱变和致癌作用。此数据库 允许对每个短期化验的能力进行评估 以预测致癌或其他短期化验结果。它还 允许对个别化验方法进行研究，包括 实验室内重复性。一项研究，以检验可预测性 四个短期测试作为短期效力的函数 检测和致癌检测反应仍在继续。这些化学品将是 评估包括先前用于关联的114种化学数据集 具有致癌性结果的定性短期检测结果，即 用于使用测试的效力来关联相同的参数 回应。这些效力的衡量标准正在开发和评估中， 并将用于相关研究。 两种计算机辅助检测沙门氏菌致突变性的预测 结构-活动系统，一个经验结构-活动系统，以及 确定了一个物化体系。测试数据库包含 从青少年测试的化学物质中随机挑选出100种化学物质 沙门氏菌的致突变性，但其结果尚未由 NTP。化学结构被绘制并发送，但没有识别 他们的名字，或编码的化学品，给参与者。该计划的目的是 这项研究有三个方面：衡量不同测试系统的准确性 与研究时的配置相同：使用以下结果 这项工作旨在确定每个系统的优点和缺点： 为结构活动预测带来理解和光明。 两个基于计算机的合成孔径雷达系统和经验系统给出了等价性 预测性(约70%-80%)，而物理化学系统不是 有效(约60%的预测性)。对结果的分析，以及 确定不同学科之间的异同之处 系统仍在继续。