TESTING OF CHEMICALS OF INTEREST IN SALMONELLA

沙门氏菌相关化学物质的测试

• 批准号: 3876946
• 负责人: E ZEIGER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3876946
• 关键词: DNA; Salmonella; Salmonella typhimurium; acidity /alkalinity; adduct; buffers; busulfan; chemical carcinogen; chemical structure function; dyes; epoxides; food contamination; furans; high performance liquid chromatography; histamine; laboratory rat; metal complex; methane sulfonate; mutagen testing; mutagens; phenobarbital; salts; toxicant interaction; toxicant screening; tryptamines; tyramine

The hair dye component, HC Blue 1 was subjected to HPLC to isolate impurities that may be responsible for its mutagenicity. The commercial dye and its mutagenic fraction, but not the HPLC-purified dye, produce DNA adducts in Salmonella. These results show that the mutagenicity of HC Blue 1 due to its impurities. The food mutagens, IQ and MeIQ, were studied following activation by S9 from different rat strains in the presence of biogenic amines. Tryptamine enhanced IQ mutagenicity by Fischer rat S9 and inhibited it with Sprague- Dawley and Wistar rat S9. Tryptamine enhanced the mutagenicity of MeIQ, regardless of the S9 source. Tyramine produced different patterns, and histamine had no effect. These patterns of interactions suggest rat strain differences in the activation of IQ and MeIQ. Preincubation of Salmonella and metal salts in distilled-deionized water resulted in the detection of mutagenic activity with cobalt chloride, ferrous sulphate, cadmium chloride, and zinc chloride; phosphate buffer, which is normally used, reduces or eliminates the mutagenicity. Magnesium and citrate in the minimal medium were also responsible for these effects. HEPES buffer does not inhibit mutagenicity and may be a good alternative to phosphate for use with bacterial and mammalian cells.

通过HPLC分离染发剂成分HC Blue 1 可能导致其致突变性的杂质。 商业广告 染料及其诱变部分（但不是 HPLC 纯化的染料）产生 DNA 沙门氏菌中的加合物。 这些结果表明 HC Blue 的致突变性 1 由于其杂质。 S9 激活后研究了食物诱变剂 IQ​​ 和 MeIQ 在生物胺存在的情况下来自不同的大鼠品系。 色胺 Fischer 大鼠 S9 增强 IQ 致突变性，Sprague- 抑制它 道利和威斯塔大鼠 S9。 色胺增强了MeIQ的致突变性， 无论 S9 来源如何。 酪胺产生不同的模式，并且 组胺没有作用。 这些相互作用模式表明大鼠应变 IQ 和 MeIQ 激活的差异。 在蒸馏去离子水中预培养沙门氏菌和金属盐 结果检测到氯化钴的诱变活性， 硫酸亚铁、氯化镉和氯化锌；磷酸盐缓冲液， 通常使用的，可以减少或消除致突变性。 镁 基本培养基中的柠檬酸盐也是造成这些影响的原因。 HEPES 缓冲液不会抑制致突变性，可能是一个很好的替代品 磷酸盐用于细菌和哺乳动物细胞。