BIO-RAD CONFOCAL MICROSCOPE & 3-D ANALYSIS WORK STATION

这项共享仪器奖助金适用于共焦显微镜和3-D 图像分析工作站。这台设备将提供一种新技术 为了推进7名NIH资助的研究人员的研究计划，开展了一项 共有9个国家卫生研究院资助的研究项目对此有很强的申请 技术的具体项目及其建议的应用 共焦成像和三维图像分析如下：1)三维 脑内输出神经元功能识别亚群的重建 细胞内注射荧光后的初级味觉核团 染料；2)亚细胞成分的可视化和形态计量学 (静毛纤毛和空泡)暴露于暂时性耳聋的毛细胞- 3)荧光逆行标记细胞的形态计量学 雏鸡心脏的完整感觉神经节和交感神经节 心脏功能不全的胚胎；4)完整角膜的成像测量 结合荧光示踪钙离子运动的体积变化 染料，以研究角膜上皮中的水和离子运输；5)成像 内源性药物在完整眼球角膜亚细胞器中的应用 线粒体氧化还原链的荧光检测 不同环境氧浓度下的角膜；6)通过 完整和不透明的角膜以评估过氧化氢的潜在有害影响 浓度对睫状突、角膜内皮细胞形态的影响 和晶状体上皮；7)发育中的胚胎心脏流出的成像 研究结构化3-D弹性矩阵的组织，使用 基质成分的荧光标记和这一过程的中断 通过中性牙冠消融；以及9)检查 细胞外基质成分的结构，到其支持能力 在尝试神经修复过程中的神经再生。 研究人员是解剖学、生理学和内分泌学系，以及 佐治亚医学院眼科学和电子工程学 佐治亚理工学院。他们和解剖学主席在一起， 部门协调支持人员，将构成一名行政人员 监督该设施使用的委员会。巴里·马斯特斯博士，他有 在共焦成像方面拥有丰富的经验，托马斯·罗森奎斯特博士是 在大学的一个共焦设施进行研究。来自南卡罗来纳州的威尔 为其他用户提供专业知识和帮助并提供技术支持 在建立这个设施方面。

This Shared Instrumentation Grant is for a confocal microscope and 3-D image analysis work station. This equipment will provide a new technology to advance the research programs of 7 NIH-funded researchers, conducting a total of 9 NIH-funded research projects with strong applications for this technology. The specific projects, and their proposed applications of confocal imaging and 3-D image analysis are as follows: 1) 3-D reconstruction of functionally-identified subgroups of output neurons in the primary gustatory nucleus after intracellular injection of fluorescent dye; 2) visualization and morphometrics of subcellular components (stereocilia and vacuoles) of hair cells exposed to temporary deafness- inducing noise; 3) morphometrics of fluorescent retrogradely-labeled cells innervating the heart in intact sensory and sympathetic ganglia, in chick embryos with cardiac disfunction; 4) imaging of intact cornea to measure volume changes in conjunction with tracking Ca++ movement with fluorescent dyes, to study water and ion transport in corneal epithelium; 5) imaging of subcellular organelles in the cornea of the intact globe using endogenous fluorescence of the mitochondrial redox chain to asses viability of the cornea under varying ambient oxygen concentrations; 6) imaging through intact and opaque corneas to asses potentially deleterious effects of H2O2 concentrations on morphology of the ciliary process, corneal endothelium and lens epithelium; 7) imaging of developing embryonic cardiac outflow tracts to study organization of the structured, 3-D elastic matrix, using fluorescent labeling of matrix components and 8) disruption of this process by neutral crest ablation; and 9) examination of the relationship of the structure of extracellular matrix constituents, to its ability to support nerve regeneration during attempted nerve repair. The investigators are faculty in Anatomy, Physiology and Endocrinology, and Ophthalmology at Medical College of Georgia, and Electrical Engineering at Georgia Institute of Technology. They with the Anatomy chairman, and the departmental confocal support person , will constitute an Executive Committee to oversee use of the facility. Dr. Barry Masters, who has extensive experience in confocal imaging, and Dr. Thomas Rosenquist, who is conducting research at a confocal facility at Univ. of South Carolina, will provide expertise and assistance to the other users and technical support in setting up the facility.