IMAGE PROCESSING OF ELECTRON MICROGRAPHS

该项目便于从电子显微镜中确定结构。 提供了适当的软件、硬件和科学专业知识 允许其他科学家，主要是NIH的科学家使用图像处理和 通过计算机重建来确定或理解标本的结构。 分析的数据类型包括中间细丝、笼状三棱柱 腿和膜结构。 显微照片是用飞利浦EM400T显微镜拍摄的 布鲁克海文体育馆。用光学衍射法预选了一些显微照片。 底片在Perkin-Elmer 1010 mg显微密度计上数字化， 通过PIC计算机系统进行分析。结果是照相写字 Perkin-Elmer显微密度计或MATRIX摄像工作站。图片 使用主要由NIH开发的软件进行处理。 软件开发将根据需要继续进行。我们预计会增加 一些3-D图像处理能力。此外，作为新的生物， 结构变得可供分析时，将对其进行检查。

This project facilitates structure determination from electron microscopy. Suitable software, hardware, and scientific expertise has been provided to allow other scientists, primarily at NIH, to use image processing and computer reconstruction to determine or understand a specimen's structure. Types of data analyzed include intermediate filaments, clathrin triskelion legs, and membrane structures. The micrographs were taken with a Philips EM400T microscope and the Brookhaven STEM. some micrographs were preselected by optical diffraction. Negatives were digitized on a Perkin-Elmer 1010MG microdensitometer and analyzed by means of the PIC computer system. Results were photowritten on the Perkin-Elmer microdensitometer or on a Matrix camera station. Images were processed using software developed primarily at NIH. Software development will continue as needed. We anticipate addition of some 3-D image processing capabilities. In addition, as new biological structures become available for analysis, these will be examined.