IMAGE PROCESSING OF ELECTRON MICROGRAPHS

该项目有助于从电子显微镜确定结构。 已经提供了适当的软件、硬件和科学专门知识， 允许其他科学家，主要是在NIH，使用图像处理， 计算机重建以确定或理解标本的结构。 分析的数据类型包括中间丝，网格蛋白三聚体， 腿和膜结构。 显微照片用Philips EM 400 T显微镜拍摄， 布鲁克海文STEM。 一些显微照片通过光学衍射预选。 在Perkin-Elmer 1010 MG显微密度计上对阴性进行数字化， 通过PIC计算机系统进行分析。 结果被影印在 Perkin-Elmer显微密度计或Matrix照相机站上。 图像 使用主要由NIH开发的软件进行处理。 软件开发将根据需要继续进行。 我们预计， 一些三维图像处理能力。 此外，作为新的生物 结构变得可用于分析，这些将被检查。

This project facilitates structure determination from electron microscopy. Suitable software, hardware, and scientific expertise has been provided to allow other scientists, primarily at NIH, to use image processing and computer reconstruction to determine or understand a specimen's structure. Types of data analyzed include intermediate filaments, clathrin triskelion legs, and membrane structures. The micrographs were taken with a Philips EM400T microscope and the Brookhaven STEM. some micrographs were preselected by optical diffraction. Negatives were digitized on a Perkin-Elmer 1010MG microdensitometer and analyzed by means of the PIC computer system. Results were photowritten on the Perkin-Elmer microdensitometer or on a Matrix camera station. Images were processed using software developed primarily at NIH. Software development will continue as needed. We anticipate addition of some 3-D image processing capabilities. In addition, as new biological structures become available for analysis, these will be examined.