VIRUS STRUCTURE AND BACTERIA FROM IMAGE PROCESSING OF ELECTRON MICROGRAPHS

电子显微照片图像处理中的病毒结构和细菌

• 批准号: 3874814
• 负责人: B L TRUS
• 金额: --
• 依托单位: CENTER FOR INFORMATION TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3874814
• 关键词: artificial intelligence; bacteria; computer program /software; computer system design /evaluation; densitometry; electron microscopy; image processing; interferometry; mathematical model; statistics /biometry; virus morphology

Software is being developed (with NIAMS and BEIP) to analyze viral and bacterial images and perform various statistical and mathematical tests. Such images are typically analyzed by Fourier filtering techniques, and the use of correlation alignment together with correlation averaging, and three-dimensional Fourier reconstruction techniques. The electron micrographs are taken with a Philips EM400T microscope, a Zeiss 902 microscope, and the Brookhaven STEM. Some micrographs are preselected by optical diffraction. Negatives are digitized on a Perkin- Elmer 1010MG microdensitometer and analyzed by means of the PIC computer system. Results are photowritten on the Perkin-Elmer microdensitometer and on a Matrix camera station. Image processing uses software developed primarily at NIH. We anticipate evaluating other viruses and bacteria for suitability for examination with these methods, and continuing with this ongoing project to determine the structure of various classes of viruses.

正在开发软件（使用NIAMS和BEIP）来分析病毒和 细菌图像，并执行各种统计和数学测试。 这样的图像通常通过傅立叶滤波技术来分析， - 使用相关对准和相关平均，以及 三维傅立叶重建技术。 电子显微照片用Philips EM 400 T显微镜拍摄， 蔡司902显微镜和布鲁克海文STEM。 一些显微照片是 通过光学衍射预选。 底片会在珀金电脑上数码化- Elmer 1010 MG显微密度计，并通过PIC计算机进行分析 系统 结果在Perkin-Elmer显微密度计上照相记录， 一个矩阵摄像站 图像处理使用开发的软件 主要是在NIH。 我们预计将评估其他病毒和细菌是否适合 用这些方法进行检查，并继续进行这一正在进行的项目， 确定各类病毒的结构。