ROLE OF SURFACE MOLECULES IN THE METABOLISM AND ECOLOGY OF ORAL BACTERIA

表面分子在口腔细菌代谢和生态中的作用

• 批准号: 3875237
• 负责人: J LONDON
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3875237
• 关键词: Actinomyces; Actinomycetales infection; Bacteroides; N acetylneuraminate; Streptococcus infection; Streptococcus sanguis; adhesin; affinity chromatography; aminoacid; aminosugar; bacterial DNA; bacterial proteins; binding proteins; carbohydrates; cell adhesion; cell adhesion molecules; complementary DNA; fibronectins; hybrid cells; laboratory mouse; laminin; lysine; mass spectrometry; membrane activity; messenger RNA; microorganism metabolism; nucleic acid probes; oral bacteria; pilus; protein sequence; proteolysis

The binding specificities of the adhesive protein associated with the fimbriae of Bacteroides loeschei (now Prevotella loeschei) that mediates coaggregation with Streptococcus oralis and agglutination of RBCs has been further characterized. Studies with 125I-labeled adhesin established that its interaction with the streptococcal cell receptor is indeed a lectin-carbohydrate interaction readily reversed by amino sugars. Interaction with the carbohydrate receptor on S. oralis, prevents the streptococcus from coaggregating with its other partner cells, i.e., Actinomyces naeslundii, Veillonella atypica and Streptococcus sanguis. However, adhesion to PBCs or RBC ghosts appears to be a protein-protein interaction. The adhesin also binds to a number of eucaryotic structural proteins including laminin and fibronectin. Limited proteolysis studies indicate that the streptococcal-specific adhesin is antigenically related to the second adhesin which mediates coaggregation between P. loeschei and Actinomyces israelii. Currently the gene encoding the streptococcal-specific adhesin is being sequenced to determine its structure; this was facilitated by obtaining an internal peptide sequence of the adhesive protein. A mRNA transcript of the gene was identified; from its size, 2.9 kb, it appears that, at least, one other protein is being synthesized concomitantly with the adhesin.

粘附蛋白的结合特异性与 Loeschei类杆菌（现为Prevotella loeschei）的菌毛，介导 与口腔链球菌的共聚集和红细胞的凝集， 进一步特征化。用125 I标记的粘附素进行的研究证实， 它与链球菌细胞受体的相互作用确实是 凝集素-碳水化合物相互作用容易被氨基糖逆转。 与S.口腔，防止 链霉菌与其其他伴侣细胞共聚集，即， 内氏放线菌、韦荣氏球菌和血链球菌。 然而，与PBMC或RBC血影的粘附似乎是一种蛋白质-蛋白质粘附。 互动粘附素还结合到许多真核结构蛋白上。 包括层粘连蛋白和纤连蛋白的蛋白质。有限的蛋白水解研究 表明链球菌特异性粘附素是抗原相关的 第二粘附素介导P. loeschei和 以色列放射菌。 目前，编码链球菌特异性粘附素的基因正在被 测序以确定其结构;这是通过获得一个 粘附蛋白的内部肽序列。一个mRNA转录本， 基因被鉴定;从它的大小，2.9 kb，似乎，至少，一个 其它蛋白质与粘附素同时合成。