MEMBRANE ASSOCIATED MOLECULES IN THE METABOLISM AND ECOLOGY OF ORAL BACTERIA

口腔细菌代谢和生态中的膜相关分子

• 批准号: 3963771
• 负责人: J LONDON
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963771
• 关键词: Actinomycetales; carbohydrate metabolism; cell aggregation; genetic strain; gram positive bacteria; host organism interaction; membrane activity; microorganism immunology; microorganism metabolism; microorganism population study; mutant; oral bacteria; periodontium disorder; virulence

The three neotype species of Capnocytophaga can be identified and classified by the specificity of the surface adhesins which are responsible for their coaggregation to various gram positive oral bacteria. The adhesin activities on the surface of Capnocytophaga ochracea were distinguished by sugar inhibition studies. Coaggregation between C. ochracea and strains of (1) Streptococcus sanguis, (2) Actinomyces viscosus - Antinomyces naeslundii and (3) Actinomyces israelii were inhibited by low concentrations of rhamnose (1mM), high concentrations of rhamnose (10mM) or combinations of rhamnose and sialic acid, respectively. Capnocytophaga sputigena coaggregates with the same strains of the A. viscosus - A. naeslundii cluster and A. israelii group as C. ochracea; these interactions are inhibited by high concentrations of rhamnose (10mM) or rhamnose and sialic acid, respectively. C. gingivalis coaggregates only with strains of A. israelii in a sialic acid sensitive interaction. Naturally occurring coaggregation defective mutants of C. ochracea were isolated which paralleled the phenotypes of C. sputigena and C. gingivalis, that is, the respective coaggregation patterns of these mutants and their sugar inhibition profiles corresponded perfectly with the two neotype species. The reciprocity exhibited by antisera prepared against C. ochracea or C. gingivalis in blocking coaggregation of both gliders with their respective partners indicates that the adhesins on these two organisms are not only functionally homologous, but that they may actually share a degree of structural homology as well. Ribitol-5-P dehydrogenase was purified to homogeneity from ribitol-grown cells of Lactobacillus casei C116 and compared to the xylitol-5-P dehydrogenase from L. casei C183. This key component of the ribitol metabolizing pathway was found to be physically and kinetically distinct from the xylitol-5-P dehydrogenase. No immunological homology could be demonstrated between the two enzymes.

三种嗜碳细胞噬菌的新类型可以被识别和鉴定