CELL ENVELOPE-ASSOCIATED MOLECULES IN ORAL BACTERIA METABOLISM

口腔细菌代谢中的细胞包膜相关分子

• 批准号: 4692584
• 负责人: J LONDON
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4692584
• 关键词: Actinomyces; Bacteroides; Enterococcus; Streptococcus mutans; Streptococcus sanguis; biochemical evolution; cell capsule; cell membrane; enzyme structure; evolution; glyceraldehyde 3 phosphate dehydrogenase; metabolism; microorganism immunology; microorganism metabolism; oral bacteria; phosphotransferases; serology /serodiagnosis

A study of the export systems of the xylitol and 2-deoxyglucose futile cycles has focused on the oepration and regulation of a phosphohydrolase that cleaves xylitol-5-P and 2 deoxyglucose-6-P, respectively, prior to the expulsion of the free sugars. The enzyme hydrolyses a number of sugar phosphates, but is specific for (1) substrates phosphorylated at the terminal carbon position, (2) substrates with a D-erythro configuration and, (3) preferentially hydrolyzes those substrates that exist in a cyclical (pyranose or furanose) configuration. Enzyme activity is regulated by a membrane-associated component which can be removed from the membrane by extensive washing in high salt solutions. This regulatory mechanism accounts for the conservation of phosphorylated intermediates during the operation of the futile cycles and, more importantly, may be responsible for maintaining a balance between the intracellular levels of Pi and phosphorylated glycolytic intermediates under all conditions of growth. The lectin-like protein responsible for coaggregation between Capnocytophaga gingivalis (emended) and Actinomyces israelii that is found on the cell envelope of the former has been identified and partially characterized. Two dimensional electrophoresis and western blot analysis used in conjunction with specifically adsorbed antisera revealed that the lectin is a basic protein (pI=i8.4) with a molecular weight of 155KDa. The protein is located exclusively on the outer member of C. gingivalis. Progress on the identification characterization of lectins found on cells of Bacteroides loeschei 1295 and Capnocytophaga ochracea stain 25 that are reponsible for coaggregation with strains of Streoptococcus sanguis and Actinomyces israelii will be summarized in Dr. Paul Kolenbrander's report.

木糖醇和2-脱氧葡萄糖非糖输出系统的研究 cycles的研究重点是磷酸水解酶的作用和调节 分别切割木糖醇-5-P和2-脱氧葡萄糖-6-P， 游离糖的排出。 这种酶水解大量的糖 磷酸盐，但特异于（1）底物磷酸化在 末端碳位置，（2）具有D-邻位构型底物 和，（3）优先水解存在于 环状（吡喃糖或呋喃糖）构型。 酶活性 由膜相关成分调节，该成分可以从膜中去除。 膜在高盐溶液中广泛洗涤。 这一监管 磷酸化中间体的保守机制 在无效循环的操作期间，更重要的是， 负责维持细胞内水平之间的平衡 Pi和磷酸化糖酵解中间体在所有条件下， 增长 凝集素样蛋白负责之间的共聚集 牙龈二氧化碳嗜纤维菌（修订）和以色列放线菌，发现 在前者的细胞包膜上已经被识别， 表征了 双向电泳和蛋白质印迹分析 与特异性吸附的抗血清结合使用显示， 凝集素是一种碱性蛋白质（pI= 18.4），分子量为155 KDa。 的 蛋白质仅位于C的外部成员上。牙龈炎 细胞表面凝集素的鉴定研究进展 Loeschei 1295和Capnocytophaga ochracea菌株25的菌株， 负责与血链球菌菌株共聚集， 将在Paul Kolenbrander博士的报告中总结以色列放线菌。