THE ROLE OF SURFACE MOLECULES IN THE METABOLISM AND ECOLOGY OF ORAL BACTERIA

表面分子在口腔细菌代谢和生态中的作用

• 批准号: 3917146
• 负责人: J LONDON
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3917146
• 关键词: Actinomyces; Actinomycetales infection; Bacteroides; Capnocytophaga; Streptococcus infection; Streptococcus sanguis; adhesin; affinity chromatography; bacterial proteins; binding proteins; cell adhesion; glucose receptor; hybrid cells; laboratory mouse; mass spectrometry; microorganism metabolism; monoclonal antibody; nuclear magnetic resonance spectroscopy; oral bacteria; pilus; protein sequence

The two fimbriae associated adhesins of Bacteroides loescheii which mediate the coaggregations with Streptococcus sanguis 34 or Actinomyces israelii PK14 are presently being characterized. The S. sanguis 34 specific adhesin appears to be a hexamer composed of 6 - 75kD subunits. Moderate quantities of this protein have been purified to homogeneity by conventional and affinity chromatography. Its N-terminal amino acid sequence and amino acid composition has been determined and its pI has been estimated. Purification and characterization of the A. israelii PK14 adhesin is also underway. A method of purify the fimbriae that carry these adhesins has been developed, these larger structures appear to be complex helices composed of four intertwined protein strands. The carbohydrate receptor on S. sanguis H1 which is recognized by an adhesin on Capnocytophaga ochracea has been isolated from streptococcal cell walls, purified to homogeneity and characterized by NMR and mass spectroscopy. It was shown to be composed of a repeating hexasaccharide composed of rham-rham-gal-gal-gal-glu linked through phosphodiester groups. Monclonal antibodies specific for an afimbriate adhesin on the outer membrane of Capnocytophaga gingivalis DR2001 have been used to identify and localize this lectin like protein. The adhesin mediates the coaggregation by C. gingivalis and strains of Actinomyces israelii. The adhesins are not found uniformly over the surface of the cell and appear to be preferentially located at the poles of the cell. Like B. loescehii, there are relatively few adhesin molecules per cell, between 190 and 300.

洛氏拟杆菌与粘附素相关的两种菌毛 介导与血链球菌34或 目前正在对以色列放线菌PK14进行鉴定。这个 血链球菌34特异性粘附素似乎是由以下组成的六聚体 6-75kD亚基。适量的这种蛋白质已经被 通过传统和亲和力纯化到同质 层析法。其N-端氨基酸序列和氨基酸序列 测定了其组成，估算了其等电点。 板蓝根霉菌PK14粘附素的纯化及性质研究 也在进行中。一种净化携带这些细菌的菌毛的方法 粘附素已经被开发出来，这些更大的结构似乎是 由四条相互缠绕的蛋白质链组成的复杂螺旋。 血链霉菌H1上的碳水化合物受体 从褐毛藻中分离到一种粘附素 链球菌细胞壁，纯化至均一，并表征 通过核磁共振谱和质谱学分析。它被证明是由一个 由大黄-大黄-半乳糖-谷氨酸组成的重复六糖 通过磷酸二酯基团连接。 抗流苏状粘附素的单克隆抗体 牙周炎细胞吞噬杆菌DR2001外膜已被使用 鉴定和定位这种凝集素样蛋白。粘附性 介导牙龈假单胞菌和牙周炎菌株的共聚集 以色列放线菌。粘附素并不是均匀分布在 电池的表面，并且看起来优先位于 细胞的两极。像B.loescehii这样的物种相对较少 每个细胞的粘附素分子，在190到300之间。