THE CONVERSION OF PHENYLALANINE TO TYROSINE

苯丙氨酸转化为酪氨酸

• 批准号: 3880871
• 负责人: S KAUFMAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3880871
• 关键词: aminoacid metabolism; enzyme induction /repression; enzyme mechanism; enzyme structure; enzyme substrate; hepatocellular carcinoma; isozymes; laboratory rat; molecular pathology; neoplastic cell culture for noncancer research; neurochemistry; phenylalanine; phenylalanine 4 monooxygenase; phosphorylation; pteridine analog; pteridines; tyrosine

Rat liver phenylalanine hydroxylase has been crystallized. A procedure has been developed for purifying to homogeneity large amounts of cloned rat liver phenylalanine hydroxylase from E.coli extracts. The physical and catalytic properties of the cloned enzyme appear to be identical to the enzyme from rat liver. A procedure has been developed for obtaining pure kidney phenylalanine hydroxylase. Isolated by this procedure, the enzyme retains its unique highly activated state. Phenylalanine in vivo induces phosphorylation-activation of rat liver phenylalanine hydroxylase.

大鼠肝苯丙氨酸羟化酶已结晶。 已开发出一种用于大量纯化至均匀的程序， 从大肠杆菌提取物中克隆大鼠肝脏苯丙氨酸羟化酶。的 克隆酶的物理和催化性质似乎是 和老鼠肝脏里的酶一样 一个程序已开发获得纯肾苯丙氨酸 羟化酶通过这种方法分离，酶保留了其独特的 高度活跃的状态。 苯丙氨酸诱导大鼠肝脏磷酸化活化 苯丙氨酸羟化酶