BLOOD-BRAIN BARRIER CHANGES FOLLOWING REPEATED ISCHEMIC INSULTS

反复缺血性损伤后血脑屏障发生变化

• 批准号: 3881803
• 负责人: T S NOWAK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3881803
• 关键词: albumins; artery occlusion; blood brain barrier; brain mapping; calcium metabolism; carotid artery; cell death; cerebral ischemia /hypoxia; cerebrovascular occlusions; electron microscopy; gerbil /jird; glia; glial fibrillary acidic protein; hippocampus; immunocytochemistry; laboratory rabbit; neurons; thalamus

Rabbit antisera have been raised against purified gerbil albumin and used for the immunocytochemical localization of the extravasated protein in brain following repeated ischemic results. A striking accumulation of albumin immunoreactivity was evident in thalamus as early as 6 hours after a series of repeated 5 minute carotid artery occlusions while positive staining was first evident in this region at 24 hours following repeated 2 minute occlusions. At longer recirculation intervals, thalamic staining was intensified and the CAl region of hippocampus showed strong immunoreactivity which accompanied the degeneration of neurons in this vulnerable area. In general, albumin immunoreactivity was closely correlated with the distribution of enhanced 45calcium 45uptake as well as with the appearance of reactive glia, as evidenced by immunocytochemical visualization of glial fibrillary acidic protein. The similarity of intraneuronal uptake of albumin to the endocytotic uptake of a protein tracer, observed in epileptic convulsions, suggests that neuroexcitatory mechanisms may be involved in uptake of proteins from the extracellular space in both conditions.

兔抗血清已被提高对纯化沙鼠白蛋白和使用 用于外渗蛋白的免疫细胞化学定位， 脑缺血反复发作的结果。惊人的积累 白蛋白免疫反应在丘脑中明显，早在6小时后， 一系列重复的5分钟颈动脉闭塞， 在重复2次后24小时，在该区域中染色首次明显 微小的闭塞在较长的再循环间隔，丘脑染色 海马CA1区呈强阳性反应， 免疫反应性，伴随着神经元的变性，在这个 脆弱的地区。总的来说，白蛋白免疫反应性与 与增强的45钙45摄取的分布相关， 免疫细胞化学证实， 胶质细胞酸性蛋白的可视化。的相似性 白蛋白的神经元内摄取到蛋白质的内吞摄取 示踪剂，观察癫痫惊厥，表明神经兴奋性 机制可能涉及从细胞外摄取蛋白质 空间在这两种情况下