COORDINATE CHANGES IN BRAIN ENERGY METABOLISM AND PROTEIN SYNTHESIS

大脑能量代谢和蛋白质合成的协调变化

• 批准号: 3945236
• 负责人: T S NOWAK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3945236
• 关键词: amphetamines; bioenergetics; brain metabolism; cerebral ischemia /hypoxia; gel electrophoresis; glycogen; glycogenolysis; heat; high performance liquid chromatography; hippocampus; immunochemistry; neuropharmacology; phosphorylation; radioimmunoassay

This long term project investigating the integral relationship between protein synthesis and other aspects of brain metabolism has provided the initial studies on which two new projects are based, dealing with stress protein induction and depletion of hippocampal dynorphin after ischemia. These are outlined in detail elsewhere in this report. The role of hyperthermia in the reduction of protein synthesis after amphetamine administration has been demonstrated in mice, and correlated with enhanced drug-induced brain glycogenolysis at higher body temperatures. Current research continues to focus on ischemia, with a more direct emphasis on the regulation of protein synthesis as such. Effects of repeated ischemic intervals on brain protein synthesis are being investigated using the gerbil model currently available in the laboratory. Other studies will evaluate the role of initiation factor phosphorylation in the characteristic lasting reduction of protein synthesis after ischemia. On a more applied level it is considered that protein synthesis activity may be used as an index of ischemic stress in the preparation of tissue slices, providing a means for optimizing brain slices which may be applicable in a variety of physiological studies.

这一长期项目研究的是整体关系 蛋白质合成与大脑新陈代谢的其他方面 提供了关于以下两个新项目的初步研究 基于，处理应激蛋白的诱导和耗尽 脑缺血后海马区强啡肽。这些在中有概述 在本报告的其他地方提供了详细信息。 高温在蛋白质合成减少中的作用 在小鼠身上证实服用安非他明后， 并与增强的药物诱导的脑糖原分解相关 较高的体温。 目前的研究继续集中在缺血上，更多的 直接强调对蛋白质合成的调节。 反复缺血间隔对脑蛋白质合成的影响 正在使用目前可用的沙鼠模型进行研究 在实验室里。其他研究将评估 启动因子磷酸化在特征持续中的作用 缺血后蛋白质合成减少。在更适用的情况下 水平，认为蛋白质合成活性可用于 作为组织切片制备中的缺血应激的指标， 提供了一种优化脑片的方法，这可能是 适用于各种生理学研究。