REGULATION OF HIPPOCAMPAL DYNORPHIN LEVELS AND SYNTHESIS AFTER ISCHEMIA

缺血后海马强啡肽水平和合成的调节

• 批准号: 3922605
• 负责人: T S NOWAK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3922605
• 关键词: brain metabolism; cerebral ischemia /hypoxia; dynorphins; gerbil /jird; hippocampus; histochemistry /cytochemistry; messenger RNA; neurochemistry; neuropeptides; neurotransmitter biosynthesis; neurotransmitter metabolism

Earlier results had demonstrated a lasting depletion of hippocampal dynorphin after ischemia in the gerbil. Immunoreactivity in hippocampal extracts remained at 50% of control levels for at least one week after a single 5 min ischemic episode. Immunocytochemical studies verify the localization of dynorphin peptides in hippocampal dentate granule cells an their mossy fiber projections to CA3. The pattern of immunoreactivity remains unchanged during recirculation after transient ischemia, suggesting that the postischemic depletion of dynorphin peptides is homogeneous throughout this cell population. Future studies will evaluate the levels and distribution of prodynorphin mRNA in hippocampus after ischemia.

早期的研究结果表明，海马神经元的持久消耗 强啡肽在沙土鼠缺血后的变化。 免疫反应性 海马提取物保持在50%的对照水平至少 在单次5分钟缺血发作后一周。 免疫细胞 研究证实了强啡肽的定位， 海马齿状回颗粒细胞及其苔藓纤维投射 到CA3。 免疫反应性的模式保持不变， 短暂缺血后再循环，提示 缺血后强啡肽的消耗 在整个细胞群体中是均匀的。 未来的研究将 评估强啡肽原mRNA的水平和分布， 海马缺血后。