REGULATION OF HIPPOCAMPAL DYNORPHIN LEVELS AND SYNTHESIS AFTER ISCHEMIA

缺血后海马强啡肽水平和合成的调节

• 批准号: 3901574
• 负责人: T S NOWAK
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3901574
• 关键词: autoradiography; brain metabolism; cerebral ischemia /hypoxia; dynorphins; genetic transcription; gerbil /jird; hippocampus; in situ hybridization; messenger RNA; neurotransmitter biosynthesis; neurotransmitter metabolism

In situ hybridization studies demonstrate a decrease in prodynorphin mRNA in dentate granule cells of gerbil hippocampus following transient ischemia. Quantitative assessment by means of grain counts following autoradiography indicate a decrease of approximately 50% at 24h recirculation, comparable to the decreases in dynorphin peptide immunoreactivity in hippocampal extracts observed in previous studies. This down regulation of dynorphin synthesis at the transcriptional level is similar to that described by other investigators following seizures, and is consistent with an increased activation of hippocampal circuitry following ischemia. In view of the concurrent induction of c-fos and hsp7O in this cell population, altered synthesis of opioid peptide precursors would appear to be another component of what is coming to be understood as a complex pathophysiological response of neurons to ischemia and other stresses.

原位杂交研究显示前啡肽mRNA减少