CONFOCAL MICROSCOPE--3-DIMENSIONAL RECONSTRUCTION

共焦显微镜--三维重建

• 批准号: 3520607
• 负责人: KALPANA P WHITE
• 金额: $ 14.1万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-22 至 1991-11-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520607
• 关键词: biomedical equipment purchase; biomedical equipment resource; microscopy

We request funds for a confocal microscope with the capability to optically section and accomplish three-dimensional reconstructions. We will establish a joint facility that will primarily serve five laboratories in the Biology Department. The proposed instruments will be used to accomplish goals of funded NIH projects. All five laboratories are involved in studies of the structure and function of arthropod nervous systems. Current research uses molecular, genetic, biochemical, electrophysiological, behavioral and conventional anatomical technologies. The proposed instrumentation will extend the precision and resolution of ongoing work, and make possible experiments that are too time-consuming using existing instruments. Major research efforts involve molecular neurogenetic studies of Drosophila development, memory, courtship, and circadian rhythms. Also included are anatomical studies of the distribution of neuroactive molecules such as peptide neurotransmitters in Drosophila and in crustacean stomatogastric nervous systems. Subcellular localizations of proteins involved in RNA processing in yeast and Drosophila neurons are also intended.

我们请求资金购买具有光学能力的共焦显微镜 截面并完成三维重建。 我们将 建立一个联合设施，主要为五个实验室提供服务 生物系。 拟议的文书将用于 实现资助的 NIH 项目的目标。 所有五个实验室均 参与节肢动物神经结构和功能的研究 系统。 目前的研究使用分子、遗传、生物化学、 电生理学、行为学和传统解剖学技术。 拟议的仪器将提高精度和分辨率 正在进行的工作，并进行可能太耗时的实验 使用现有仪器。 主要研究工作涉及分子 果蝇发育、记忆、求偶和发育的神经遗传学研究 昼夜节律。 还包括解剖学研究 神经活性分子（例如肽类神经递质）的分布 果蝇和甲壳动物的口胃神经系统。 亚细胞 参与酵母 RNA 加工的蛋白质的定位 果蝇神经元也是有意的。