ELAV AND NEURON SPECIFIC ALTERNATIVE SPLICING

ELAV 和神经元特异性选择性剪接

• 批准号: 2892219
• 负责人: KALPANA P WHITE
• 金额: $ 28.89万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2892219
• 关键词: DNA footprinting; Drosophilidae; RNA binding protein; RNA biosynthesis; RNA splicing; gel mobility shift assay; genetic regulation; messenger RNA; neurons; polymerase chain reaction; protein sequence; protein structure function

DESCRIPTION: The elav gene plays an essential role in the developing nervous system of Drosophila. The elav protein was cloned and characterized molecularly by the White lab a number of years ago. It was shown to be expressed specifically within the nervous system and selectively within neuronal nuclei. The Elav protein has a modular structure comprising 3 C-terminal RNA binding domain (RBD) motifs, a tethering sequence between the second and 3rd domain and an N-terminal auxiliary domain. Particular interest has centered on the RBD domains since they raise the intriguing notion that Elav may participate in the control of neuro-specific RNA biogenesis or stability. RBD domains in the Drosophila sex lethal protein play an important role in regulating sex-specific splicing in flies and several RBD-containing proteins in mammalian cells have been implicated in controlling RNA stability. Recent studies from the White lab provide strong evidence that the Elav protein is both necessary and sufficient for the neuron-specific splicing of the cell surface protein neuroglian. Although the phenomenon of neuron-specific splicing was first described over a decade ago no transacting neuron-specific factors have been identified. Elav represents an excellent candidate for a neuron-specific splicing factor. This proposal is designed to analyze the function of elav in neuron-specific splicing.

描述：elav基因在发育中起着重要作用。 果蝇的神经系统 对ELAV蛋白进行了克隆和鉴定 白色实验室在几年前用分子的方式研究了它。 它被证明是 在神经系统中特异性表达， 神经细胞核 Elav蛋白具有模块化结构，其包含3个 C-末端RNA结合结构域（RBD）基序，即RNA结合结构域之间的系链序列。 第二和第三结构域以及N端辅助结构域。 特别 兴趣集中在RBD领域，因为它们提出了有趣的 Elav可能参与控制神经特异性RNA的概念 生物起源或稳定性。 果蝇性致死蛋白的RBD结构域 在调节果蝇性别特异性剪接中发挥重要作用， 哺乳动物细胞中的几种含RBD的蛋白质与 控制RNA稳定性。 白色实验室最近的研究提供了强有力的证据， 证据表明，Elav蛋白是必要的和足够的， 细胞表面蛋白神经胶质细胞的神经元特异性剪接。 虽然 神经元特异性剪接现象在十多年前首次被描述， 以前还没有确定出交易神经元特异性因子。 埃拉夫 代表了神经元特异性剪接因子的优秀候选者。 本研究旨在分析elav在神经元特异性 拼接