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ELAV AND NEURON SPECIFIC ALTERNATIVE SPLICING

ELAV 和神经元特异性选择性剪接

基本信息

批准号：
6393869
负责人：
KALPANA P WHITE
金额：
$ 23.13万
依托单位：
BRANDEIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-05-01 至 2003-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6393869
关键词：
DNA footprinting Drosophilidae RNA binding protein RNA biosynthesis RNA splicing gel mobility shift assay genetic regulation messenger RNA neurons polymerase chain reaction protein sequence protein structure function

项目摘要

DESCRIPTION: The elav gene plays an essential role in the developing nervous system of Drosophila. The elav protein was cloned and characterized molecularly by the White lab a number of years ago. It was shown to be expressed specifically within the nervous system and selectively within neuronal nuclei. The Elav protein has a modular structure comprising 3 C-terminal RNA binding domain (RBD) motifs, a tethering sequence between the second and 3rd domain and an N-terminal auxiliary domain. Particular interest has centered on the RBD domains since they raise the intriguing notion that Elav may participate in the control of neuro-specific RNA biogenesis or stability. RBD domains in the Drosophila sex lethal protein play an important role in regulating sex-specific splicing in flies and several RBD-containing proteins in mammalian cells have been implicated in controlling RNA stability. Recent studies from the White lab provide strong evidence that the Elav protein is both necessary and sufficient for the neuron-specific splicing of the cell surface protein neuroglian. Although the phenomenon of neuron-specific splicing was first described over a decade ago no transacting neuron-specific factors have been identified. Elav represents an excellent candidate for a neuron-specific splicing factor. This proposal is designed to analyze the function of elav in neuron-specific splicing.

描述： elav 基因在发育中起着至关重要的作用果蝇的神经系统。 elav 蛋白被克隆并表征几年前，怀特实验室从分子角度研究了这一点。结果显示是在神经系统内特异性表达并选择性地在神经元核。 Elav 蛋白具有包含 3 个模块的模块结构 C 端 RNA 结合域 (RBD) 基序，是第二和第三结构域以及 N 端辅助结构域。特别的人们的兴趣集中在 RBD 领域，因为它们提出了有趣的问题 Elav 可能参与神经特异性 RNA 的控制生物发生或稳定性。果蝇性致死蛋白中的 RBD 结构域在调节果蝇性别特异性剪接中发挥重要作用哺乳动物细胞中的几种含有 RBD 的蛋白质与控制RNA稳定性。怀特实验室最近的研究提供了强有力的证据有证据表明，Elav 蛋白对于细胞表面蛋白神经胶质细胞的神经元特异性剪接。虽然神经元特异性剪接现象在十多年前首次被描述在此之前，尚未发现神经元特异性的影响因素。埃拉夫代表神经元特异性剪接因子的优秀候选者。该提案旨在分析 elav 在神经元特异性中的功能拼接。