ELAV AND NEURON SPECIFIC ALTERNATIVE SPLICING

ELAV 和神经元特异性选择性剪接

• 批准号: 2038908
• 负责人: KALPANA P WHITE
• 金额: $ 21.81万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2038908
• 关键词: DNA footprinting; Drosophilidae; RNA binding protein; RNA biosynthesis; RNA splicing; gel mobility shift assay; genetic regulation; messenger RNA; neurons; polymerase chain reaction; protein sequence; protein structure function

DESCRIPTION: The elav gene plays an essential role in the developing nervous system of Drosophila. The elav protein was cloned and characterized molecularly by the White lab a number of years ago. It was shown to be expressed specifically within the nervous system and selectively within neuronal nuclei. The Elav protein has a modular structure comprising 3 C-terminal RNA binding domain (RBD) motifs, a tethering sequence between the second and 3rd domain and an N-terminal auxiliary domain. Particular interest has centered on the RBD domains since they raise the intriguing notion that Elav may participate in the control of neuro-specific RNA biogenesis or stability. RBD domains in the Drosophila sex lethal protein play an important role in regulating sex-specific splicing in flies and several RBD-containing proteins in mammalian cells have been implicated in controlling RNA stability. Recent studies from the White lab provide strong evidence that the Elav protein is both necessary and sufficient for the neuron-specific splicing of the cell surface protein neuroglian. Although the phenomenon of neuron-specific splicing was first described over a decade ago no transacting neuron-specific factors have been identified. Elav represents an excellent candidate for a neuron-specific splicing factor. This proposal is designed to analyze the function of elav in neuron-specific splicing.

描述：elav基因在病毒的发育过程中起着至关重要的作用。 果蝇的神经系统。克隆并鉴定了elav蛋白。 几年前由怀特实验室在分子上进行了研究。它被证明是 特异性地在神经系统内表达，并选择性地在 神经元核团。Elav蛋白具有模块结构，包括3 C-末端RNA结合域(RBD)基序，是 第二和第三结构域和N端辅助结构域。特例 人们的兴趣集中在RBD领域，因为它们引发了有趣的 认为Elav可能参与神经特异性RNA的控制 生物发生或稳定性。果蝇性致死蛋白中的RBD结构域 在调节果蝇性别特异性剪接方面发挥重要作用 哺乳动物细胞中的几种含有RBD的蛋白质被认为与 控制RNA的稳定性。来自白宫实验室的最新研究提供了强有力的证据 有证据表明Elav蛋白对于 神经元特异性的细胞表面蛋白神经胶质细胞的剪接。虽然 神经元特异性剪接现象是十多年来首次描述的。 在此之前，还没有发现与神经元相关的特定因子。伊拉夫 代表了神经元特异性剪接因子的极佳候选者。 这项建议旨在分析elav在神经元特异性中的功能。 拼接。