ELAV AND NEURON SPECIFIC ALTERNATIVE SPLICING

ELAV 和神经元特异性选择性剪接

• 批准号: 2038908
• 负责人: KALPANA P WHITE
• 金额: $ 21.81万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2038908
• 关键词: DNA footprinting; Drosophilidae; RNA binding protein; RNA biosynthesis; RNA splicing; gel mobility shift assay; genetic regulation; messenger RNA; neurons; polymerase chain reaction; protein sequence; protein structure function

DESCRIPTION: The elav gene plays an essential role in the developing nervous system of Drosophila. The elav protein was cloned and characterized molecularly by the White lab a number of years ago. It was shown to be expressed specifically within the nervous system and selectively within neuronal nuclei. The Elav protein has a modular structure comprising 3 C-terminal RNA binding domain (RBD) motifs, a tethering sequence between the second and 3rd domain and an N-terminal auxiliary domain. Particular interest has centered on the RBD domains since they raise the intriguing notion that Elav may participate in the control of neuro-specific RNA biogenesis or stability. RBD domains in the Drosophila sex lethal protein play an important role in regulating sex-specific splicing in flies and several RBD-containing proteins in mammalian cells have been implicated in controlling RNA stability. Recent studies from the White lab provide strong evidence that the Elav protein is both necessary and sufficient for the neuron-specific splicing of the cell surface protein neuroglian. Although the phenomenon of neuron-specific splicing was first described over a decade ago no transacting neuron-specific factors have been identified. Elav represents an excellent candidate for a neuron-specific splicing factor. This proposal is designed to analyze the function of elav in neuron-specific splicing.

描述：Elav基因在发展中起着至关重要的作用 果蝇神经系统。 elav蛋白被克隆并表征 几年前，白人实验室的分子。 被证明是 专门在神经系统中表达，在 神经元核。 Elav蛋白具有一个模块化结构，包括3 C末端RNA结合结构域（RBD）基序，一个束缚序列 第二和第三域以及N末端辅助域。 特别的 兴趣已经集中在RBD域，因为它们引起了人们的关注 Elav可能参与神经特异性RNA的观念 生物发生或稳定性。 果蝇性致死蛋白中的RBD域 在调节苍蝇中的性别特定剪接方面发挥重要作用 哺乳动物细胞中的几种含RBD的蛋白已与 控制RNA稳定性。 White Lab的最新研究提供了强大的 伊拉夫蛋白既需要又足够的证据 细胞表面蛋白神经胶质的神经元特异性剪接。 虽然 神经元特异性剪接的现象首先描述了十年 以前未鉴定出交易神经元特异性因素。 Elav 代表神经元特异性剪接因子的出色候选者。 该建议旨在分析ELAV在神经元特异性中的功能 剪接。