ANALYSIS OF GENES ESSENTIAL FOR NEURONAL DIFFERENTIATION
神经元分化必需基因的分析
基本信息
- 批准号:6240444
- 负责人:
- 金额:$ 14.53万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-07-01 至 1998-06-30
- 项目状态:已结题
- 来源:
- 关键词:Drosophilidae RNA binding protein RNA splicing amyloid proteins biological signal transduction cell differentiation confocal scanning microscopy developmental genetics developmental neurobiology gene deletion mutation gene expression genetic regulatory element genetically modified animals immunocytochemistry neurogenetics neurons protein structure function receptor reporter genes tissue /cell culture
项目摘要
The proposed experiments will elucidate functions of two pan-
neurally expressed proteins, ELAV and APPL, in differentiation
and maintenance of all neurons. The long term objective of studies
related to ELAV is to understand mechanism of neuron-specific
alternative splicing. Mechanisms of neuron-specific splicing will
be investigated using ELAV, a neuron-specific RNA binding
protein, and it~s downstream identified target, the regulated intron
of the gene neuroglian. Specifically, these studies will elucidate
the mechanism of neuron-specific alternative splicing of the nrg
pre-mRNA by defining cis-elements involved in the regulated
intron that bind ELAV and other nuclear factors, and assess their
in vivo significance. Other neuron-specific factors that regulate
this process will be identified. These studies will use reporter
transgenes in vivo and in cell culture assays to assess splicing,
biochemical approaches to study RNA-protein interactions, and
genetic methods to identify other factors. The Drosophila APPL
protein is a member of the beta amyloid precursor protein (APP)
family of proteins. The first identified member of this family,
APP, is implicated to have a role in Alzheimer~s disease.
Although this family of proteins is widely expressed within the
nervous system, the normal physiological roles of these proteins
are not well understood. The hypothesis that the APPL protein in
Drosophila neuron function sas a cell surface receptor to regulate
neuronal processes and synapses will be tested. Other proteins
that are involved in APPL-mediated processes will be identified.
The experimental design will make extensive use of molecular and
genetic approaches possible in Drosophila: use of wild type and in
vitro mutagenized transgenes and transgenic animals, and targeted
expression, to assay in vivo function. Immunocytochemical
techniques and confocal microscopy will be used to study neuronal
arbors and neurophysiological studies will be conducted study
synaptic function. Since ELAV and APPL both belong to
evolutionarily conserved protein families this analysis will
contribute to the mechanistic understanding of the function of
related proteins in humans.
所提出的实验将阐明两种泛函的功能
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('KALPANA P WHITE', 18)}}的其他基金
Request for Leica TCS SP2 Spectral Confocal Microscope
索取 Leica TCS SP2 光谱共焦显微镜
- 批准号:
6440990 - 财政年份:2002
- 资助金额:
$ 14.53万 - 项目类别:
ANALYSIS OF GENES ESSENTIAL FOR NEURONAL DIFFERENTIATION
神经元分化必需基因的分析
- 批准号:
6481919 - 财政年份:2001
- 资助金额:
$ 14.53万 - 项目类别:
ANALYSIS OF GENES ESSENTIAL FOR NEURONAL DIFFERENTIATION
神经元分化必需基因的分析
- 批准号:
6325868 - 财政年份:2000
- 资助金额:
$ 14.53万 - 项目类别:
ANALYSIS OF GENES ESSENTIAL FOR NEURONAL DIFFERENTIATION
神经元分化必需基因的分析
- 批准号:
6107521 - 财政年份:1999
- 资助金额:
$ 14.53万 - 项目类别:
ANALYSIS OF GENES ESSENTIAL FOR NEURONAL DIFFERENTIATION
神经元分化必需基因的分析
- 批准号:
6296690 - 财政年份:1998
- 资助金额:
$ 14.53万 - 项目类别:
ANALYSIS OF GENES ESSENTIAL FOR NEURONAL DIFFERENTIATION
神经元分化必需基因的分析
- 批准号:
6271746 - 财政年份:1998
- 资助金额:
$ 14.53万 - 项目类别:
ELAV AND NEURON SPECIFIC ALTERNATIVE SPLICING
ELAV 和神经元特异性选择性剪接
- 批准号:
2038908 - 财政年份:1997
- 资助金额:
$ 14.53万 - 项目类别:
ELAV AND NEURON SPECIFIC ALTERNATIVE SPLICING
ELAV 和神经元特异性选择性剪接
- 批准号:
6393869 - 财政年份:1997
- 资助金额:
$ 14.53万 - 项目类别:
ELAV AND NEURON SPECIFIC ALTERNATIVE SPLICING
ELAV 和神经元特异性选择性剪接
- 批准号:
2892219 - 财政年份:1997
- 资助金额:
$ 14.53万 - 项目类别:
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