Request for Leica TCS SP2 Spectral Confocal Microscope

索取 Leica TCS SP2 光谱共焦显微镜

• 批准号: 6440990
• 负责人: KALPANA P WHITE
• 金额: $ 41.1万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440990
• 关键词: biomedical equipment purchase; confocal scanning microscopy; neurophysiology; protein localization

DESCRIPTION (provided by applicant): We request funds for the purchase of a Leica Spectral Confocal Microscope (Leica TCS SP2 UV). Our current BlO-RAD 600 microsocope is 10 years old and no longer adequate for current standards of research. Brandeis University does not currently possess any other confocal imaging facility. In fact a state of the art confocal is absolutely essential to the N.I.H. funded research of the user group. It will also benefit other life science researchers at Brandeis interested in imaging biological material. Of the ten investigators in the user group, seven are current users and the other three are investigators whose research is now well poised to take advantage of confocal imaging. Projects include: mechanisms of synaps formation, synaptic plasticity, neuromodulation, regulation of neurotransmitter release, mechanisms underlying development of neuronal diversity, localization of biological rhythm associated proteins, localization of sexual behavior associated proteins, mechanisms in RNA processing and transport, and mechanisms of intracellular organelle transport. The Leica TCS2 SP2 instrument would be housed in the Imaging Facility that currently houses our old confocal. Because we have had a confocal facility for 10 years, mechanisms of operation and maintenance are already in place. The Imaging facility is managed by an "Imaging Specialist" who also oversees the day to day operation of the confocal microsope including training of new users and maintenance of the instrument. Brandeis University and the Biology Department are committed to maintain this facility. In addition to the user group, the confocal facility will be available to all life science researchers at Brandeis who have a need for confocal imaging on a time-available basis.

描述（由申请人提供）：我们要求购买资金 Leica光谱共聚焦显微镜（Leica TCS SP2 UV）。我们目前的Blo-Rad 600 微型ocopope已有10年的历史，并且不再足以满足当前标准 研究。布兰代斯大学目前没有其他共同点 成像设施。实际上，最终的共识状态绝对是必不可少的 到N.I.H.资助用户组的研究。它也将受益其他 Brandeis的生命科学研究人员对成像生物材料感兴趣。 在用户组的十个调查人员中，有七个是当前用户， 其他三个是研究人员，他们的研究现在已经有好准备 共聚焦成像的优势。项目包括：突触机制 形成，突触可塑性，神经调节，神经递质的调节 释放，神经元多样性发展的机制，本地化 与生物节奏相关蛋白质，性行为的定位 相关蛋白质，RNA加工和运输中的机制以及机制 细胞内细胞器转运。 Leica TCS2 SP2仪器将容纳在成像设施中 目前是我们的旧共聚焦。因为我们有一个共同的设施 10年，已经有了操作和维护机制。这 成像设施由“成像专家”管理，他还监督 共聚焦微压的日常操作，包括对新用户的培训 和仪器的维护。布兰代斯大学和生物学 部门致力于维护此设施。除了用户 小组，共聚焦设施将为所有生命科学研究人员提供 在布兰代斯（Brandeis），他们需要在时间上进行共聚焦成像。