Request for Leica TCS SP2 Spectral Confocal Microscope

描述(由申请人提供)：我们申请资金购买 徕卡光谱共聚焦显微镜(徕卡TCS SP2 UV)。我们目前的BLO-RAD 600 微粒显微镜已有10年的历史，不再适合当前的标准 研究。布兰代斯大学目前没有任何其他共焦 成像设备。事实上，最先进的共焦技术是绝对必要的 国家卫生研究院资助的用户群体研究。它还将使其他人受益 布兰迪斯大学的生命科学研究人员对生物材料成像感兴趣。 在用户组的10名调查员中，有7名是现任用户， 其他三人是调查人员，他们的研究现在已经做好了准备 共焦成像的优势。项目包括：同步机制 形成、突触可塑性、神经调节、神经递质调节 释放，神经元多样性发展的潜在机制，定位 生物节律相关蛋白，性行为的定位 相关蛋白质、RNA加工和运输的机制以及机制 细胞内细胞器的运输。 徕卡TCS2 SP2仪器将安装在成像设施中， 目前存放着我们的旧共焦相机。因为我们有一个共焦设备 10年来，运行和维护机制已经到位。这个 成像设施由一名“成像专家”管理，该专家还负责监督 共焦显微镜的日常操作，包括培训新用户 以及仪器的维护。布兰迪斯大学与生物学 各部门都致力于维护这个设施。除了用户之外 集团，共焦设施将向所有生命科学研究人员开放 在布兰代斯，他们需要在有时间的基础上进行共焦成像。

DESCRIPTION (provided by applicant): We request funds for the purchase of a Leica Spectral Confocal Microscope (Leica TCS SP2 UV). Our current BlO-RAD 600 microsocope is 10 years old and no longer adequate for current standards of research. Brandeis University does not currently possess any other confocal imaging facility. In fact a state of the art confocal is absolutely essential to the N.I.H. funded research of the user group. It will also benefit other life science researchers at Brandeis interested in imaging biological material. Of the ten investigators in the user group, seven are current users and the other three are investigators whose research is now well poised to take advantage of confocal imaging. Projects include: mechanisms of synaps formation, synaptic plasticity, neuromodulation, regulation of neurotransmitter release, mechanisms underlying development of neuronal diversity, localization of biological rhythm associated proteins, localization of sexual behavior associated proteins, mechanisms in RNA processing and transport, and mechanisms of intracellular organelle transport. The Leica TCS2 SP2 instrument would be housed in the Imaging Facility that currently houses our old confocal. Because we have had a confocal facility for 10 years, mechanisms of operation and maintenance are already in place. The Imaging facility is managed by an "Imaging Specialist" who also oversees the day to day operation of the confocal microsope including training of new users and maintenance of the instrument. Brandeis University and the Biology Department are committed to maintain this facility. In addition to the user group, the confocal facility will be available to all life science researchers at Brandeis who have a need for confocal imaging on a time-available basis.