MOLECULAR ANALYSIS OF THE OM MUTATOR IN DROSOPHILA ANANASSAE

果蝇 OM 突变体的分子分析

• 批准号: 3941555
• 负责人: C H LANGLEY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3941555
• 关键词: animal population genetics; eye; gel electrophoresis; gene expression; genetic manipulation; genetic mapping; genetic regulation; linkage mapping; molecular cloning; sex chromosomes; transposon /insertion element

Drosophila ananassae is a species characterized by several unusual genetic phenomena: hypermutability, meiotic male crossing over and a transposable element, tom, that often jumps causing a specific and unusual mutant phenotype, Om (dominant eye morphology). DNA sequences homologous to the tom element and the target sites of insertion have been analyzed. The tom element is a retrovirus-like transposon that is similar to the 297 element of D. melanogaster. The target site of insertion of the tom element is TATAT. The ATAT portion of the target is duplicated upon insertion. Many other features of typical retroviruses are found in the structure of the tom element. The analysis of a set of Om mutants at particular locus, Om(1D), indicates that the mutant phenotype is associated with the insertion of the tom element(s) in a number of sites in a region greater than 25 kilobases. Spontaneous revertants are due to the excision of tom elements.

果蝇（Drosophila ananassae）是一个具有几个特征的物种， 不寻常的遗传现象：超变，减数分裂男性 交换和一个转座因子tom 导致一种特殊的和不寻常的突变表型，Om（显性 眼形态学）。 与汤姆的DNA序列同源 元件和插入的靶位点进行了分析。 tom元件是一种类似逆转录病毒的转座子， D. 297元素黑腹菌 插入的目标部位 tom元素的第一个元素是TATAT。 目标的ATAT部分 在插入时被复制。 典型的许多其他特征 逆转录病毒存在于TOM元件的结构中。 的 对特定位点Om（1D）上的一组Om突变体进行分析， 表明突变表型与 将TOM元件插入区域中的多个位置 大于25个的酶。 自发回复突变是由于 切除Tom元件。