MOLECULAR ANALYSIS OF THE OM MUTATOR IN DROSOPHILA ANANASSAE

果蝇 OM 突变体的分子分析

• 批准号: 4693260
• 负责人: C H LANGLEY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4693260
• 关键词: animal population genetics; eye; gel electrophoresis; gene expression; gene mutation; genetic manipulation; genetic mapping; genetic regulation; linkage mapping; molecular cloning; nucleic acid sequence; sex chromosomes; transposon /insertion element

Hinton described an unusual mutator in D. ananassae which he speculated was a transposable element, tom, with (previously) novel properties: the element could only be detected phenotypically by its effect on eye morphology at 21 non-pleiotropic, non-dosage compensted, non-random loci. Because of the recovery of a spontaneous singed mutation, sn9g, in an ocullar morphology (Om) mutant line derivative, Om(lD)9g, it was possible to investigate his speculation. D. melanogaster singed, sn, DNA probes were used to isolate and recover a D. ananassae singed gene. A comparison of the sn9g and wild type singed restriction map implicated a 6.5 kb insert as the element responsible for the mutator effect. A total of 186 recombinant lines from four X-linked Om loci were examined. 80 were Om and 106 were non-Om; in all instances an in situ hybridization signal, when probed with sn9g insert, was found at appropriate locations on the polytene chromosomes. Linkage was complete and showed that the sn9g insert was homologous to sequences localized at the sites of Om mutants. Preliminary analysis of several isolated clones of the tom element indicate a fairly conserved structure typical of copia-like elements. DNA sequence analysis of the tom at sn9g and Om(lD)9g showed direct repeats at the terminai that is also characteristic of copia-like elements. On going southern blot studies of several OM(lD) mutations indicate that some are due to insertions of a single tom, others appear to be due to multiple copies and yet others show no obvious alterations in the immedite vicinity of Om(lD)9g. Further investigations of the tom elements and the Om mutations should provide insight into the mechanisms underlying this very unusual mutational process.

欣顿在D.他推测这是 一个转座因子，tom，具有（以前）的新特性： 元素只能通过其对眼睛的影响进行表型检测 21个非多效性、非剂量补偿、非随机位点的形态学。 由于在一个人的染色体上发现了一个自发的突变sn 9 g， Om突变株系衍生物Om（ID）9 g， 调查他的推测 D.黑腹鱼，sn，DNA探针 分离并回收了一株D. ananassae烧焦基因。 的比较 sn 9 g和野生型的限制性内切酶切图谱显示了一个6.5kb的插入片段 作为负责增变效应的元素。 共有186 检查了来自四个X连锁的Om基因座的重组系。 80个是Om， 106例为非Om;在所有情况下，当 在聚乙烯上的适当位置发现sn 9 g插入物 染色体 连接是完整的，并表明sn 9 g插入物是 与位于Om突变体位点的序列同源。 对几个分离的tom分子克隆的初步分析表明， 一种相当保守的copia样元件的典型结构。 DNA序列 对sn 9 g和Om（1D）9 g处的tom的分析显示，在sn 9 g和Om（1D）9 g处存在直接重复。 也是copia类元素的特征。 要去 对几种OM（ID）突变的Southern印迹研究表明， 由于插入的是单个汤姆，其他的似乎是由于多个汤姆。 副本和其他副本显示在immedite附近没有明显的变化 的Om（ID）9 g。 tom元素和Om的进一步研究 突变应该提供深入了解这一机制的基础， 不寻常的突变过程