Development of leucocyte cell lines for immunological research in teleost fish

用于硬骨鱼免疫学研究的白细胞系的开发

• 批准号: G0800725/1
• 负责人: Jun Zou
• 金额: $ 32.43万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0800725%2F1
• 关键词: Development; leucocyte; cell; lines; immunological

White blood cells, or leucocytes, are the cell that defence the body from infections and provide disease resistance. Research into the function of leucocytes is greatly aided in mammals by the availability of many leucocyte cell lines, that allow a lot of research to be performed using culture systems without the need to harvest tissues and cells from animals. These developments have been possible in large part by the discovery of many leucocyte growth factors, that can be produced using molecular methodologies, and added to the cultures as growth supplements. In this proposal we aim to develop leucocyte cell lines for fish, due to increasing use of fish by researchers, partly as a consequence of the major expansion of aquaculture and its associated needs (eg fish health issues). The applicants are internationally renown for their work on the fish equivalents of the leucocyte growth factors, and so can produce relevant proteins to encourage leucocytes to grow in vitro. Several different approaches will be taken to generate the cell lines, and some preliminary characterisation is proposed, so that they will quickly become a valuable resource for researchers. In addition to a reduction in the numbers of fish being used, a reduction in fish suffering should also follow this development, since the cell lines will also allow preliminary in vitro testing of compounds and chemicals to see if they affect leucocyte function, before testing in live fish.

白细胞或白细胞是防御人体免受感染并提供抗病性的细胞。通过许多白细胞细胞系的可用性，可以在哺乳动物中进行对白细胞功能的研究，从而可以使用培养系统进行大量研究，而无需从动物那里收集组织和细胞。这些发展在很大程度上是通过发现许多白细胞生长因子，这些因素可以使用分子方法论产生，并将其作为生长补充剂添加到培养物中。在此提案中，由于研究人员对鱼类的使用越来越多，我们旨在开发鱼类细胞系，部分原因是水产养殖及其相关需求的重大扩展（例如鱼类健康问题）。申请人因其在白细胞生长因子的鱼类等效因素上的工作而在国际上享有盛誉，因此可以产生相关蛋白质以鼓励白细胞在体外生长。将采用几种不同的方法来生成细胞系，并提出了一些初步表征，以便它们很快成为研究人员的宝贵资源。除了减少所使用的鱼类数量外，鱼类苦难的减少还应遵循这种发育，因为细胞系还将允许对化合物和化学物质的初步体外测试，以查看它们是否影响白细胞功能，然后在活鱼中进行测试。