Development of leucocyte cell lines for immunological research in teleost fish

用于硬骨鱼免疫学研究的白细胞系的开发

• 批准号: G0800725/1
• 负责人: Jun Zou
• 金额: $ 32.43万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0800725%2F1
• 关键词: Development; leucocyte; cell; lines; immunological

White blood cells, or leucocytes, are the cell that defence the body from infections and provide disease resistance. Research into the function of leucocytes is greatly aided in mammals by the availability of many leucocyte cell lines, that allow a lot of research to be performed using culture systems without the need to harvest tissues and cells from animals. These developments have been possible in large part by the discovery of many leucocyte growth factors, that can be produced using molecular methodologies, and added to the cultures as growth supplements. In this proposal we aim to develop leucocyte cell lines for fish, due to increasing use of fish by researchers, partly as a consequence of the major expansion of aquaculture and its associated needs (eg fish health issues). The applicants are internationally renown for their work on the fish equivalents of the leucocyte growth factors, and so can produce relevant proteins to encourage leucocytes to grow in vitro. Several different approaches will be taken to generate the cell lines, and some preliminary characterisation is proposed, so that they will quickly become a valuable resource for researchers. In addition to a reduction in the numbers of fish being used, a reduction in fish suffering should also follow this development, since the cell lines will also allow preliminary in vitro testing of compounds and chemicals to see if they affect leucocyte function, before testing in live fish.

白细胞，或白细胞，是保护身体免受感染和提供疾病抵抗力的细胞。许多白细胞细胞系的存在极大地帮助了对哺乳动物白细胞功能的研究，这使得许多研究可以使用培养系统进行，而无需从动物身上获取组织和细胞。这些发展在很大程度上是由于发现了许多白细胞生长因子，这些因子可以用分子方法产生，并作为生长补充剂添加到培养物中。在本提案中，我们的目标是为鱼类开发白细胞细胞系，因为研究人员越来越多地使用鱼类，部分原因是水产养殖的大规模扩张及其相关需求（例如鱼类健康问题）。申请人因其在鱼类白细胞生长因子方面的工作而享誉国际，因此可以产生相关蛋白质以促进白细胞在体外生长。将采用几种不同的方法来产生细胞系，并提出一些初步的特征，以便它们将迅速成为研究人员的宝贵资源。除了减少使用的鱼的数量，减少鱼的痛苦也应该随着这一发展而减少，因为细胞系也将允许在活体鱼测试之前，对化合物和化学物质进行初步的体外测试，看看它们是否影响白细胞功能。