APPLICATION OF RAPID FLOW MICROFLUOROMETRY TO CELL BIOLOGY

快速流动显微荧光测定法在细胞生物学中的应用

• 批准号: 3962943
• 负责人: J R WUNDERLICH
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3962943
• 关键词: B lymphocyte; T lymphocyte; antibody specificity; automated data processing; cell biology; cell cell interaction; cell differentiation; chemical binding; chemotherapy; drug tolerance; flow cytometry; gene expression; genetic strain; human tissue; immunogenetics; immunoglobulins; immunotherapy; interleukin 2; major histocompatibility complex; membrane activity; monoclonal antibody; neoplasm /cancer genetics; neoplasm /cancer immunology; peripheral blood vessel; spleen; surface antigens

Using rapid flow microfluorometry (FMF) for analysis and sorting of cells, aspects of the following projects have been supported during the previous year: (1) the expression of IL 2 receptors on normal and malignant cells and on cells from donors treated with IL 2 or undergoing graft-versus-host disease, (2) the characterization of epidermal cells involved with generating immune responses, and (3) analysis of cells transfected with MHC genes. In addition, a user-interactive set of programs has been added to the flow cytometry laboratory to help data processing and presentation.

使用快速流动显微荧光测定法（FMF）分析和分选细胞， 以下项目的各个方面在前几年得到了支持： 年：（1）正常和恶性肿瘤细胞IL 2受体表达 以及来自用IL 2处理或经历移植物抗宿主的供体的细胞 疾病，（2）涉及表皮细胞的表征 产生免疫应答，和（3）分析用MHC转染的细胞 基因. 此外，还增加了一套用户交互式程序， 流式细胞仪实验室，以帮助数据处理和演示。