APPLICATION OF RAPID FLOW MICROFLUOROMETRY TO CELL BIOLOGY

快速流动显微荧光测定法在细胞生物学中的应用

• 批准号: 4691758
• 负责人: J R WUNDERLICH
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4691758
• 关键词: B lymphocyte; T lymphocyte; antibody specificity; automated data processing; cell biology; cell cell interaction; cell differentiation; chemical binding; chemotherapy; drug tolerance; flow cytometry; gene expression; genetic strain; human tissue; immunogenetics; immunoglobulins; immunotherapy; interleukin 2; major histocompatibility complex; membrane activity; monoclonal antibody; peripheral blood vessel; spleen; surface antigens

Using rapid flow microfluorometry (FMF) for analysis and sorting of cells, aspects of the following projects have been supported during the previous year: (1) stoichiometry and kinetics of cytotoxic cell binding to target cells, (2) effects of human recombinant IL 2 therapy on peripheral blood lymphocytes, (3) characterization of the specificities of monoclonal antibodies against major histocompatibility complex (MHC) determinants, (4) function of transfected MHC genes, and (5) monitoring the effectiveness of in vivo immune cell depletion or reconstitution associated with various types of therapy. In addition, the capabilities of the FMF facility have been expanded by 1) increasing the number of parameters which can be analyzed on each cell, (2) adding a flow cytometer analyzer, and (3) increasing the availability and capability of data processing.

使用快速流动显微荧光测定法（FMF）进行细胞分析和分选， 以下项目的各个方面在前几年得到了支持： 年：（1）细胞毒性细胞与靶标结合的化学计量和动力学 人重组白细胞介素2治疗对外周血白细胞的影响 淋巴细胞，（3）单克隆抗体特异性的表征 抗主要组织相容性复合体（MHC）决定簇的抗体，（4） 转染的MHC基因的功能，和（5）监测的有效性， 体内免疫细胞耗竭或重建与各种 治疗的类型。 此外，FMF设施的能力具有 通过1）增加可以被 对每个细胞进行分析，（2）添加流式细胞仪分析仪，和（3） 增加了数据处理的可用性和能力。