APPLICATION OF FLOW CYTOMETRY TO CELL BIOLOGY

流式细胞术在细胞生物学中的应用

• 批准号: 3916402
• 负责人: J R WUNDERLICH
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916402
• 关键词: T lymphocyte; biomedical resource; cell biology; cell type; cellular immunity; flow cytometry; graft versus host disease; histocompatibility antigens; human tissue; immunochemistry; immunodeficiency; immunogenetics; membrane activity; single cell analysis; tissue /cell culture; tissue /cell preparation

The Experimental Immunology Branch flow cytometry laboratory currently supports 60 individual research projects for 45 investigators. These investigations involve quantitative single cell analysis of parameters associated with cell types freshly prepared from different species/tissues, as well as spectrum of in vitro cultured cells. Basic research support is provided to members of the EIB and the Immunology Branch, as well as to other members of DCBD. Currently supported projects include a broad range of applications in the areas of cellular immunology, immunochemistry, transplantation biology, clinical immunology, immunogenetics, tumor immunology, and molecular immunology. These include, but are not limited to, the following studies: a) in vivo and in vitro analyses of intra-cellular signalling via T cell surface molecules, b) characterization of T cells from animals with genetic or induced immune dysfunction; c) studies of the pathogenesis of graft-versus-host disease; d) analyses of the correlations between T cell memory and the quantitative expression of cell surface adhesion molecules; e) investigations of T cell ontogeny and differentiation; and f) analyses of the mechanisms involved in marrow graft rejection versus acceptance.

实验免疫学分会流式细胞仪实验室 目前支持60个个人研究项目，共45个 调查人员。这些调查涉及定量的单一调查 新鲜细胞类型相关参数的细胞分析 由不同物种/组织制备，以及In的光谱 体外培养的细胞。基础研究支持提供给 欧洲投资银行和免疫学分会的成员以及其他 DCBD的成员。目前支持的项目包括广泛的 在细胞免疫学领域的一系列应用， 免疫化学、移植生物学、临床免疫学、 免疫遗传学、肿瘤免疫学和分子免疫学。这些 包括但不限于以下研究：a)体内研究 以及通过T细胞进行细胞内信号传递的体外分析 表面分子，b)来自动物的T细胞的特性 遗传性或诱导性免疫功能障碍；c)研究 移植物抗宿主病的发病机制；d)分析 T细胞记忆与数量表达的相关性 细胞表面黏附分子的研究；e)T细胞的研究 个体发育和分化；以及f)机制分析 与骨髓移植排斥反应和接受反应有关。