CELL MEDIATED CYTOTOXICITY

细胞介导的细胞毒性

• 批准号: 4691748
• 负责人: J R WUNDERLICH
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4691748
• 关键词: T lymphocyte; cell cell interaction; cell differentiation; cell mediated cytotoxicity; cellular immunity; density gradient ultracentrifugation; genetic regulation; genetic strain; growth factor; immunogenetics; immunosuppression; immunotoxicity; interleukin 2; neoplasm /cancer genetics; neoplasm /cancer immunology; neoplastic cell; poly IC; radiotracer; sarcoma; sedimentation; spleen; tissue /cell culture

Broadly reactive antitumor cytotoxic cell responses, generated in vitro by mouse splenocytes cultured with the lymphokine IL2, are modulated in a strain-dependent fashion by addition of polyinosinic acid (Poly I) to the cultures. Although Poly I is a synthetic polynucleotide, macromolecular polyanionic carbohydrates, some of which occur naturally in man, have been identified which act like Poly I. Studies of mouse strains whose splenocytes generate only low levels of antitumor cytotoxicity with Poly I, show that suppressive cell-cell interactions contribute to the low responses.

广泛反应性抗肿瘤细胞毒性细胞反应，在体外由 与淋巴因子IL 2一起培养的小鼠脾细胞， 应变依赖性的方式，通过添加聚肌苷酸（聚I）， cultures. 虽然Poly I是一种合成的多核苷酸， 聚阴离子碳水化合物，其中一些天然存在于人体内， 类似于Poly I。 小鼠品系的研究， 脾细胞对Poly I仅产生低水平的抗肿瘤细胞毒性， 显示抑制性细胞-细胞相互作用有助于降低 应答