TESTING OF CHEMICALS OF INTEREST IN SALMONELLA

沙门氏菌相关化学物质的测试

• 批准号: 3965271
• 负责人: E ZEIGER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3965271
• 关键词: Salmonella typhimurium; acetylaminofluorene; alkyl nitrile; alkylating agents; antioxidants; azides; benzopyrenes; chemical carcinogen; chemical structure function; diethylstilbestrol; genetic strain; glutathione; halobiphenyl /halotriphenyl compound; hydrazines; mutagen testing; mutagens; nitrosamines; nitrosourea; occupational hazard; phenanthrene; polychlorodibenzofuran; pyrenes; sodium; toxicant screening; toxicology

Chemicals of interest have been tested for mutagenicity in Salmonella. The chemicals studied were sodium bisulfite, cyclic nitrosamines, glutathione, N-substituted phenanthreneimines, and p-nitrophenyl pentadienal (NPPD: "spy dust"). The mutagenicity of bisulfite appears to occur through a free-radical mediated mechanism, and the level of mutagenesis is dependent on the rate of autooxidation of the bisulfite ion. The mutagenicity of the nitrosamines in the presence of homogenates from various organs does not appear to be related to their organ specificity for carcinogenesis. Glutathione is mutagenic in Salmonella as a result of its metabolism by purified Gamma-glutamyl transferase. The mutagenicity of phenanthreneimines is related to the electron-attracting or releasing properties of the N-substituted moieties, and their alkylation via carbonium ions is inversely proportional to their mutagenicity. NPPD ("spy dust") and one of its metabolites is mutagenic in Salmonella. The mutagenicity appears to be dependent on nitroreduction by either bacterial or rodent liver nitroreductases.

已在沙门氏菌中检测了相关化学品的致突变性。 的 研究的化学品是亚硫酸氢钠，环亚硝胺，谷胱甘肽， N-取代菲亚胺和对硝基苯基异戊烯醛（NPPD：“spy 灰尘”）。 亚硫酸氢盐的致突变性似乎是通过 自由基介导的机制，和诱变的水平是依赖于 对亚硫酸氢盐离子的自氧化速率的影响。 的致突变性 亚硝胺在各种器官匀浆存在下， 似乎与它们对致癌作用的器官特异性有关。 谷氨酸在沙门氏菌中具有致突变性，这是由于其通过 纯化的γ-谷氨酰转移酶。 的致突变性 菲亚胺与吸电子或放电子有关， N-取代部分的性质，以及它们通过 碳离子与其致突变性成反比。 NPPD（“间谍 灰尘”），其代谢产物之一在沙门氏菌中具有致突变性。 的 致突变性似乎取决于硝酸还原， 或啮齿动物肝脏硝基还原酶。