FIBER OPTIC PROBES

需要一种合适的装置来直接测量氧分压。 用于临床和研究的血液和组织中的压力(PO2) 申请。目前可用于测量氧合不足的方法 方便性、可靠性、速度和与许多情况的相关性 利息。开发电子传感器的努力并未取得成功。 希望有一个非常小的PO2传感器，它可以插入到 几乎不受干扰的血管或组织，它将提供 短时间或长时间的瞬时和电流PO2监测 一段时间。光纤传感器是这种应用的理想选择，具有 对于生理用途，其优点是非常小的尺寸和灵活性， 安全、低成本。已开发出一种PO2传感器，该传感器基于 氧猝灭荧光的原理。这项计划的可行性 传感器及其良好的性能已经在体外得到了验证。 和活体试验，在前几年的工作中。在前一年， 作为针尖探头的传感器的开发已经完成，存在的问题 解决了保质期和稳定性的问题。 在过去的一年里，与开发一种可用的 解决了仪器和测头的问题，并将系统用于实验 测量狗眼睛中的氧分压。这一点是在与 PO2的极谱电极测量。这些实验是为了 评估该传感器在糖尿病视网膜病变研究中的适用性。

A suitable device is needed for the direct measurement of oxygen partial pressure (PO2) in blood and tissue for both clinical and research applications. Methods currently available for measuring PO2 lack convenience, reliability, speed, and relevance to many situations of interest. Efforts to develop electrical sensors have not been successful. It is desirable to have a very small PO2 sensor which can be inserted into a blood vessel or tissue with little disturbance, and which will provide instantaneous and current PO2 monitoring for either short or extended periods of time. A fiber optic sensor is ideal for this application, with the advantage, for physiological use, of very small size and flexibility, safety, and low cost. A PO2 sensor has been developed, based upon the principle of fluorescence quenching by oxygen. The feasibility of the sensor and its satisfactory performance has been demonstrated in in vitro and in vivo tests, in work in preceding years. In the previous year, the development of the sensor as a needle probe was accomplished, and problems of storage life and stability were resolved. During the past year, several problems relating to development of a usable instrument and probe were solved, and the system was used for experiments to measure PO2 in the eyes of dogs. This was done in comparison with polarographic electrode measurements of PO2. The experiments were done to evaluate the suitability of the sensor for studies of diabetic retinopathy.