ETIOLOGY OF ALLERGIC REACTIONS IN PLATELETPHERESIS DONORS

在1984年2月至7月的6个月期间，600名捐赠者中有6名 在Fenwal CS-3000细胞分离机上进行自动血小板采集 机器出现的急性反应与即刻的超敏反应一致- 活跃型反应。这项研究的目的是进一步 描述这些反应的性质，并确定具体的 过敏原(S)负责。适合皮肤测试的提取物将是 由CS-3000分离机的各种塑料器皿部件制成 基特。将对所有经历过的捐赠者进行皮肤测试 血小板采集术中的急性反应及20例重复血小板 未发生过敏反应的供者和20名健康的非捐赠者 控制。发生过敏反应的捐赠者将受到挑战 随后在CS-3000上进行分离程序，并对 血浆和尿液组胺水平。供体和对照来源的外周血细胞 血液嗜碱性粒细胞将在体外进行组胺释放测试，以回应 至CS-3000塑料器皿提取物，供体和对照血清将进行检测 在放射过敏吸附试验(RAST)中检测特异性IgE的存在 针对CS-3000软件套件的各种化学成分。 通过这种方式，我们将试图勾勒出最近这一事件的原因 我们的血小板捐献者群体中的一系列反应。

During the six month period from February to July 1984, 6 out of 600 donors undergoing automated plateletpheresis on the Fenwal CS-3000 cell separator machine developed acute reactions consistent with immediate hypersensi- tivity-type responses. The purpose of this study is to further characterize the nature of these reactions and to identify the specific allergen(s) responsible. Extracts suitable for skin testing will be prepared from the various plasticware components of the CS-3000 apheresis kit. Skin testing will be performed on all the donors who experienced acute reactions during plateletpheresis as well as on 20 repeat platelet donors not experiencing allergic reactions, and on 20 healthy non-donor controls. Donors who have had allergic reactions will be challenged by a subsequent apheresis procedure on the CS-3000, with serial quantitation of plasma and urinary histamine levels. Donor and control-derived peripheral blood basophils will be tested in vitro for histamine release in response to CS-3000 plasticware extracts, and donors and control sera will be tested in a radioallergosorbent test (RAST) for the presence of specific IgE directed against various chemical components of the CS-3000 software kits. In this manner, we shall attempt to delineate the cause of this recent cluster of reactions in our platelet donor population.