ETIOLOGY OF ALLERGIC REACTIONS IN PLATELETPHERESIS DONORS

血小板去除术供者过敏反应的病因

• 批准号: 3853080
• 负责人: S F LEITMAN
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3853080
• 关键词: allergens; basophils; blood donor; cell sorting; ethers; granulocyte; immunoglobulin E; immunologic assay /test; immunologic skin test; plateletpheresis

In February, 1984, the DTM converted from manual to automated platelet collection techniques, acquiring for this purpose four automated cell separation devices. During the next 7 years, 18 donors undergoing apheresis procedures on the Fenwal CS-3000 device experienced acute hypersensitivity reactions. Ten reactions occurred during plateletpheresis and 9 reactions occurred during granulocytapheresis procedures. The purpose of this study was to identify the allergen responsible for these reactions. Using a combination of skin tests, radioallergosorbent tests, and basophil histamine release assays, specific IgE- mediated sensitization to ethylene oxide, a gas used to sterilize the plastic disposable apheresis kits, was found in 8 of 10 plateletpheresis donors and 7 of 9 granulocytapheresis donors experiencing reactions, but in none of 140 non- reacting controls. It is estimated that as many as 1.0% of all repeat apheresis donors may become sensitized to ethylene oxide and experience allergic symptoms during donation. Two of the 9 individuals experiencing reactions during granulocyte donations had positive skin tests to hydroxyethyl starch, and appeared to become sensitized to this red cell sedimenting agent during apheresis. Although none of these reactions have been life-threatening, the DIM is continuing to test apheresis donors for sensitization to ethylene oxide and hydroxyethyl starch, and to defer sensitized individuals from subsequent CS-3000 donations.

1984年2月，DTM由手动转换为自动血小板 收集技术，为此目的获得四个自动化单元 分离装置 在接下来的7年里，18名捐赠者接受了 Fenwal CS-3000设备上的单采程序发生急性 超敏反应 血小板单采过程中发生10例反应 9例发生在粒细胞单采过程中。 的 这项研究的目的是确定过敏原负责这些 反应. 结合皮肤测试，放射性过敏吸附测试， 和嗜碱性粒细胞组胺释放试验，特异性IgE介导的致敏 转化为环氧乙烷，一种用于对塑料一次性单采进行灭菌的气体 10例单采血小板献血者中8例，9例献血者中7例 粒细胞单采术供体出现反应，但140例非粒细胞单采术供体中无一例发生反应。 反应控制。 据估计，多达1.0%的所有重复 单采供体可能对环氧乙烷敏感， 献血时的过敏症状 九个人中有两个 粒细胞捐献期间的反应有阳性皮肤试验， 羟乙基淀粉，似乎对这种红细胞变得敏感 沉淀剂。 虽然这些反应都没有危及生命，但DIM是 继续检测单采供体对环氧乙烷的致敏性， 羟乙基淀粉，并推迟致敏个体从随后的 CS-3000捐款。