MECHANISM OF CELLULAR INFILTRATION OF THE EYE IN ANTERIOR UVEITIS

前葡萄膜炎眼部细胞浸润的机制

• 批准号: 5203262
• 负责人: F ROBERGE
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5203262
• 关键词: chemoattractants; cytokine; disease /disorder model; exudate /transudate; inflammation; laboratory rat; neutrophil; pathology; uveitis

The subcutaneous injection of bacterial endotoxin in Lewis rats produces an acute intraocular inflammation evolving over a 24-hour period. This endotoxin-induced uveitis (EIU) is characterized by a biphasic protein exudation and a cellular infiltrate composed of macrophages and polymorphonuclear neutrophils (PMN). We used this model to study the mechanism of cellular infiltration in peripheral organs. EIU was induced with an injection of LPS (Salmonella typhimurium) at 350 fg/kg. The level of cytokine-induced neutrophil chemoattractant (CINC) was measured every two hours in the serum and in the aqueous humor of the eye. The kinetics of CINC production in the systemic circulation showed a rapid rise, peaking two hours after LPS injection, followed by a progressive decline over the following eight hours. In the eye, the CINC levels increased above the serum levels 10 hours after EIU induction, corresponding to the time of cellular infiltration. The inhibition of leukocyte infiltration by 56 and 64 percent with the antiadhesion molecule Ab 1B6 (2mg/rat) was accompanied by only a slight reduction in the aqueous humor CINC levels of nine and 16 percent respectively, indicating that the intraocular CINC was produced by ocular cells. The specific effect of CINC in the eye was confirmed when a direct intraocular injection of 250 ng of purified CINC was followed by a significant PMN infiltration, in the absence of protein exudation. The data demonstrate that the production of chemotactic factors by ocular tissue participates in the inflammatory reaction induced by peripheral LPS injection.

刘易斯大鼠皮下注射细菌内毒素 在24小时内产生急性眼内炎症 期这种内毒素诱导的葡萄膜炎（EIU）的特征是 双相蛋白渗出和细胞浸润， 巨噬细胞和多形核中性粒细胞（PMN）。我们用这个 研究外周血淋巴细胞浸润机制的模型 机关EIU用LPS（沙门氏菌）注射诱导 鼠伤寒沙门氏菌）。苦参碱诱导的中性粒细胞水平 每两小时测量血清中的化学引诱物（CINC）， 在眼睛的水状液中。CINC产生的动力学 体循环迅速上升，两小时后达到峰值， LPS注射，随后在以下时间内进行性下降 八个小时。在眼睛中，CINC水平升高至高于血清 EIU诱导后10小时的水平，对应于 细胞浸润56对白细胞浸润的抑制作用 抗粘附分子Ab 1B 6（2 mg/只）组为64%， 伴随着房水CINC的轻微降低 分别为9%和16%，表明 眼内CINC由眼细胞产生。的具体效果 当直接眼内注射 250 ng纯化的CINC后， 在没有蛋白质渗出的情况下的浸润。数据 证明了眼部趋化因子的产生 组织参与外周诱导的炎症反应 LPS注射。