ROLE OF INTERLEUKIN 10 IN ENDOTOXIN INDUCED UVEITIS

白细胞介素 10 在内毒素引起的葡萄膜炎中的作用

• 批准号: 5203263
• 负责人: F ROBERGE
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5203263
• 关键词: antiinflammatory agents; cellular pathology; cytokine; disease /disorder model; eye infections; immune system; laboratory mouse; leukocytes; proteins; recombinant DNA; uveitis

This project studies the role of interleukin-10 (IL-10) in uveitis. Uveitis was induced in mice by subcutaneous injection of bacterial endotoxin. Intraocular inflammation was assessed by leukocyte count and protein concentration measurement in the aqueous humor. Mouse recombinant IL-10 or its vehicle alone were administered by repeated intravenous injections. In four of five experiments, the cellular infiltration in the eye was significantly reduced. The number of cells was reduced by 62 percent, 64 percent, 48 percent, 11 percent, and 40 percent, respectively, in the group treated with IL-10 compared with the control group. IL-10 did not affect protein exudation. We conclude that systemic administration of IL-10 has an anti- inflammatory effect acting on the cellular component of the immune response in endotoxin-mediated uveitis.

本项目研究白细胞介素-10（IL-10）在葡萄膜炎中的作用。 通过皮下注射细菌性葡萄膜炎诱导小鼠葡萄膜炎 内毒素通过白细胞计数评估眼内炎症 和房水中的蛋白质浓度测量。鼠标 重组IL-10或其单独的载体通过重复给药， 静脉注射 在五个实验中的四个中，眼睛中的细胞浸润是 大幅减少。 细胞数量减少了62%， 64%，48%，11%和40%， IL-10治疗组与对照组相比。IL-10 不影响蛋白质渗出。 我们的结论是，IL-10的全身给药具有抗- 作用于免疫细胞组分的炎症作用 内毒素介导的葡萄膜炎的反应。