Using spatial statistics and genomics to develop epidemiologically relevant definitions of insecticide resistance in African Malaria Vectors

利用空间统计和基因组学制定非洲疟疾媒介中杀虫剂抗药性的流行病学相关定义

• 批准号: MR/P02520X/1
• 负责人: Martin James Donnelly
• 金额: $ 64.1万
• 依托单位: Liverpool School of Tropical Medicine
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FP02520X%2F1
• 关键词: Using; spatial; statistics; genomics; develop

In the last 15 years 260 million clinical cases of malaria have been prevented in Sub Saharan Africa, overwhelmingly through the use of insecticides that target the mosquito vector. Insecticide resistance is therefore a major threat to the sustained control of malaria. Whole genome sequencing of malaria mosquitoes has the power to revolutionise our understanding of the evolution of insecticide resistance; allowing us to design new insecticides and to develop strategies that delay the onset of resistance. However genomic technologies will only reach their full potential with improved sampling design and when extensive individual and population level metadata (collection location, infection/ disease state etc) accompany each sequenced sample. For the problem of insecticide resistance in the major malaria vector Anopheles gambiae a deep understanding of the biology lies with SSA scientists whilst the skills to sequence and analyse the data are held by centres in the North. This foundation proposal is designed to draw together these two groups with support from a world-leading spatial statistics group around the key issue of insecticide resistance evolution. Our vision is to further develop the programme to a Pan-African level, eventually devolving leadership to our SSA partners who will have the skills and experience to sustain the network.

在过去15年中，撒哈拉以南非洲地区预防了2.6亿例疟疾临床病例，主要是通过使用针对蚊子媒介的杀虫剂。因此，抗药性是持续控制疟疾的主要威胁。疟疾蚊子的全基因组测序有能力彻底改变我们对杀虫剂抗性进化的理解;使我们能够设计新的杀虫剂并制定延迟抗性发生的策略。然而，基因组技术只有在改进采样设计，并在广泛的个体和群体水平元数据（收集位置，感染/疾病状态等）伴随每个测序样本时才能充分发挥其潜力。关于主要疟疾病媒冈比亚按蚊的杀虫剂抗药性问题，撒哈拉以南非洲的科学家对生物学有着深刻的理解，而北方的中心掌握着测序和分析数据的技能。该基金会提案旨在将这两个群体聚集在一起，并得到世界领先的空间统计小组的支持，围绕杀虫剂抗药性演变的关键问题。我们的愿景是进一步发展该方案，使其达到泛非水平，最终将领导权移交给我们的撒哈拉以南非洲伙伴，他们将拥有维持该网络的技能和经验。

项目成果

Widespread occurrence of copy number variants and fixation of pyrethroid target site resistance in Anopheles gambiae (s.l.) from southern Côte d'Ivoire.

• DOI: 10.1016/j.crpvbd.2023.100117
• 发表时间: 2023
• 期刊: CURRENT RESEARCH IN PARASITOLOGY & VECTOR-BORNE DISEASES
• 作者: Kouame, Ruth M A;Lynd, Amy;Kouame, Jackson K I;Vavassori, Laura;Abo, Kouabenan;Donnelly, Martin J;Edi, Constant;Lucas, Eric
• 通讯作者: Lucas, Eric

CRISPR/Cas9 modified An. gambiae carrying kdr mutation L1014F functionally validate its contribution in insecticide resistance and interaction with metabolic enzymes

CRISPR/Cas9 修饰 An。

• DOI: 10.1101/2021.04.20.440595
• 发表时间: 2021
• 作者: Grigoraki L

Resistance to pirimiphos-methyl in West African Anopheles is spreading via duplication and introgression of the Ace1 locus.

• DOI: 10.1371/journal.pgen.1009253
• 发表时间: 2021-01
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Grau-Bové X;Lucas E;Pipini D;Rippon E;van 't Hof AE;Constant E;Dadzie S;Egyir-Yawson A;Essandoh J;Chabi J;Djogbénou L;Harding NJ;Miles A;Kwiatkowski D;Donnelly MJ;Weetman D;Anopheles gambiae 1000 Genomes Consortium
• 通讯作者: Anopheles gambiae 1000 Genomes Consortium

The genetic architecture of target-site resistance to pyrethroid insecticides in the African malaria vectors Anopheles gambiae and Anopheles coluzzii.

• DOI: 10.1111/mec.15845
• 发表时间: 2021-11
• 期刊: MOLECULAR ECOLOGY
• 影响因子: 4.9
• 作者: Clarkson, Chris S.;Miles, Alistair;Harding, Nicholas J.;O'Reilly, Andrias O.;Weetman, David;Kwiatkowski, Dominic;Donnelly, Martin J.
• 通讯作者: Donnelly, Martin J.

Education and Socio-economic status are key factors influencing use of insecticides and malaria knowledge in rural farmers in Southern Côte d'Ivoire.

• DOI: 10.1186/s12889-022-14446-5
• 发表时间: 2022-12-28
• 期刊: BMC PUBLIC HEALTH
• 影响因子: 4.5
• 作者: Kouame, Ruth M. A.;Guglielmo, Federica;Abo, Kouabenan;Ouattara, Allassane F.;Chabi, Joseph;Sedda, Luigi;Donnelly, Martin J.;Edi, Constant
• 通讯作者: Edi, Constant