Spinal cord repair from endogenous stem cells in the spinal niche

脊髓微环境中的内源干细胞修复脊髓

• 批准号: MR/R001049/1
• 负责人: Catherina Becker
• 金额: $ 29.23万
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FR001049%2F1
• 关键词: Spinal; cord; repair; endogenous; stem

Spinal cord injury in humans leads to permanent loss of function. This is despite the presence ofstem cell like cells in the spinal cord. In contrast, zebrafish regain full swimming capacity after alesion and their spinal stem cells make new neurons that contribute to the repair.Here we plan to identify the signals acting on the stem cells in the fish to then use them to improvethe reaction of stem cells of humans (in a dish) and spinal cord repair in rat models. In the courseof the project, we will develop tools that will aid our research, but also contribute to the work of ourcolleagues in the community: We will develop small proteins that can be used to drive stem cellsinto a repair type, we will adapt a new microscopy technique to monitor repair in live fish and ratsas it happens, and we will develop new stem cell lines directly from human spinal cord material.This project will identify repair factors that could then be taken into clinical trials. Our teamcomprises colleagues from the UK, Germany, Belgium, France and Poland, among them basicneuroscientists and neurosurgeons.

人类脊髓损伤导致永久性功能丧失。尽管在脊髓中存在类似干细胞的细胞。相反，斑马鱼在损伤后恢复了完全的游泳能力，它们的脊髓干细胞产生新的神经元，有助于修复。在这里，我们计划识别作用于鱼类干细胞的信号，然后用它们来改善人类干细胞的反应（在培养皿中）和大鼠模型的脊髓修复。在这个项目的过程中，我们将开发工具，这将有助于我们的研究，但也有助于我们的同事在社区的工作：我们将开发小的蛋白质，可以用来驱动干细胞进入修复类型，我们将采用一种新的显微镜技术来监测活鱼和老鼠的修复，我们将直接从人类脊髓材料中开发新的干细胞系。2这个项目将鉴定修复因子，然后将其用于临床试验。我们的团队由来自英国、德国、比利时、法国和波兰的同事组成，其中包括基础神经科学家和神经外科医生。

项目成果

CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury.

• DOI: 10.1371/journal.pgen.1009515
• 发表时间: 2021-04
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Keatinge M;Tsarouchas TM;Munir T;Porter NJ;Larraz J;Gianni D;Tsai HH;Becker CG;Lyons DA;Becker T
• 通讯作者: Becker T

Axon Regeneration - Methods and Protocols

轴突再生 - 方法和方案

• DOI: 10.1007/978-1-0716-3012-9_15
• 发表时间: 2023
• 作者: Drake L

Regenerative neurogenesis: the integration of developmental, physiological and immune signals.

• DOI: 10.1242/dev.199907
• 发表时间: 2022-04-15
• 期刊: Development (Cambridge, England)

Coaxing stem cells to repair the spinal cord.

诱导干细胞修复脊髓。

• DOI: 10.1126/science.abe1661
• 发表时间: 2020
• 期刊: Science (New York, N.Y.)
• 作者: Becker CG