NANOARRAYER: A TOOL FOR CREATING NANO-SCALE BIOARRAYS

纳米阵列：创建纳米级生物阵列的工具

• 批准号: 6139591
• 负责人: Eric R HENDERSON
• 金额: $ 10万
• 依托单位: BIOFORCE NANOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6139591
• 关键词: atomic force microscopy; biomedical equipment development; biotechnology; intermolecular interaction; microarray technology; miniature biomedical equipment; nanotechnology; technology /technique development

The ultimate goal of this research program is the development and commercialization of an ultraminiaturized, ultrasensitive, solid state, molecular analysis platform technology. This "NanoArray" technology is based on the direct interrogation of molecular interactions events in nanometer scale spatial arrays in real time without the use of any extrinsic label or molecular reporter system. It has broad ranging applications in molecular diagnostics, genomics, proteomics, and drug discovery with significant advantages in terms of reduction in materials costs and waste generated, increased throughput and sensitivity, and conceptual simplicity. Two components of the NanoArray technology will be developed. In Phase I, a prototype NanoArrayer will be constructed and used to create spatial arrays of molecules in nanometer scale addressable domains. The milestone goal of Phase I is to use this NanoArrayer to create a 10x10 array of a single molecular species and a 4x4 array of 4 different molecular species. In Phase lI the focus will shift to construction and testing of a NanoArray reader based on the principles of Atomic Force Microscopy (AFM) and improvements in the NanoArrayer design and NanoArray construction methodology. PROPOSED COMMERCIAL APPLICATIONS: Commercial applications of this technology include; high throughput screening of ligand/target interactions on solid state arrays, DNA-protein interaction screening, and solid state molecular diagnostics.

该研究计划的最终目的是超显着，超敏感，固态，分子分析平台技术的开发和商业化。这项“纳米阵列”技术基于实时对纳米量表空间阵列中分子相互作用事件的直接询问，而无需使用任何外部标签或分子报告基因系统。它在分子诊断，基因组学，蛋白质组学和药物发现中具有广泛的应用，在材料成本的降低和废物的降低，增加的吞吐量和敏感性以及概念上的简单性方面具有显着优势。将开发两个纳米阵列技术的组成部分。在第一阶段，将构建原型纳米阵列并用于在纳米尺度可寻址域中创建分子的空间阵列。第一阶段的里程碑目标是使用该纳米阵列创建单个分子物种的10x10阵列和4种不同分子物种的4x4阵列。在LI阶段，重点将根据原子力显微镜（AFM）的原理以及纳米阵列设计和纳米arraray构造方法的改进转移到纳米阵列读取器的构建和测试。拟议的商业应用：该技术的商业应用包括；在固态阵列，DNA-蛋白相互作用筛选和固态分子诊断上的配体/靶标相互作用的高吞吐量筛选。