NANOARRAYER: A TOOL FOR CREATING NANO-SCALE BIOARRAYS

纳米阵列：创建纳米级生物阵列的工具

• 批准号: 6139591
• 负责人: Eric R HENDERSON
• 金额: $ 10万
• 依托单位: BIOFORCE NANOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6139591
• 关键词: atomic force microscopy; biomedical equipment development; biotechnology; intermolecular interaction; microarray technology; miniature biomedical equipment; nanotechnology; technology /technique development

The ultimate goal of this research program is the development and commercialization of an ultraminiaturized, ultrasensitive, solid state, molecular analysis platform technology. This "NanoArray" technology is based on the direct interrogation of molecular interactions events in nanometer scale spatial arrays in real time without the use of any extrinsic label or molecular reporter system. It has broad ranging applications in molecular diagnostics, genomics, proteomics, and drug discovery with significant advantages in terms of reduction in materials costs and waste generated, increased throughput and sensitivity, and conceptual simplicity. Two components of the NanoArray technology will be developed. In Phase I, a prototype NanoArrayer will be constructed and used to create spatial arrays of molecules in nanometer scale addressable domains. The milestone goal of Phase I is to use this NanoArrayer to create a 10x10 array of a single molecular species and a 4x4 array of 4 different molecular species. In Phase lI the focus will shift to construction and testing of a NanoArray reader based on the principles of Atomic Force Microscopy (AFM) and improvements in the NanoArrayer design and NanoArray construction methodology. PROPOSED COMMERCIAL APPLICATIONS: Commercial applications of this technology include; high throughput screening of ligand/target interactions on solid state arrays, DNA-protein interaction screening, and solid state molecular diagnostics.

这项研究计划的最终目标是开发和商业化一种超小型化、超灵敏、固态的分子分析平台技术。这种“纳米阵列”技术是基于在纳米尺度空间阵列中实时直接询问分子相互作用事件，而不使用任何外部标记或分子报告系统。它在分子诊断、基因组学、蛋白质组学和药物发现方面具有广泛的应用，在减少材料成本和产生的废物、提高吞吐量和灵敏度以及概念简单方面具有显着优势。纳米阵列技术的两个组成部分将被开发。在第一阶段，将构建一个原型NanoArrayer，并用于在纳米尺度的可寻址域中创建分子的空间阵列。第一阶段的里程碑式目标是使用NanoArrayer创建单个分子物种的10x10阵列和4个不同分子物种的4x4阵列。在第一阶段，重点将转移到基于原子力显微镜（AFM）原理的纳米阵列阅读器的构建和测试，以及纳米阵列设计和纳米阵列构建方法的改进。拟议的商业应用：该技术的商业应用包括；在固体阵列上高通量筛选配体/靶标相互作用，dna -蛋白质相互作用筛选和固体分子诊断。