Microfabricated Deposition Tools for Creating NanoArrays

用于创建纳米阵列的微加工沉积工具

• 批准号: 7107512
• 负责人: Eric R HENDERSON
• 金额: $ 40万
• 依托单位: BIOFORCE NANOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7107512
• 关键词: biomaterial development /preparation; biotechnology; method development; miniature biomedical equipment; nanotechnology

DESCRIPTION (provided by applicant): The goal of this SBIR Phase II proposal is to continue and expand the development of microfabricated surface patterning tools (SPTs) for the construction of ultra miniaturized biomaterial arrays (nanoarrays). SPTs can be configured as either pre-loaded molecular ink cartridges analogous to those used in desktop printers, or as an ultraminiaturized version of the pin tool print head in a conventional microarr ayer. SPTs are a consumable component of an instrument we have constructed called NanoArrayer (with support from NIH R44 EB00316). They will also be configured to be compatible with commercial atomic force microscopes. In the proposed Phase II program we will focus on increasing the number of individual spots c reated in a single print cycle while maintaining a high degree of r eproducibility and minimal sample cross contamination. We will optimize linear array (1-D) SPTs containing 10 individually loaded cantilevers and then expand in a second dimension, culminating i n a planar 2-D print head for simultaneous printing of up to 100 nanoarray spots, each capable of containing a unique biomolecular species. E mphasis will be given to controlling sample spil lover (cross-contamination), spot size, printing repr oducibility and SPT-surface alignment. We will also begin to explore forward looking development in the areas of localized magnetic field cantilever control, alternative (non-microcantilever) SPT designs and soft polymer print substrates that can be used for microfluidics and for nanoar ray cloning via contact printing. At the end of the Phase II program we will have an ensemble of STP-based solutions for a variety of ultramicro- and nanoarray applications. Our commer cialization plan will continue throughout the Phase II development program with introduction of improved SPTs as they emerge from the R&D process.

描述（由申请人提供）：SBIR第二阶段提案的目标是继续和扩大用于构建超小型化生物材料阵列（纳米阵列）的微制造表面图案化工具（spt）的发展。spt可以配置为类似于桌面打印机中使用的预加载分子墨盒，也可以配置为传统微层中pin工具打印头的超小型化版本。spt是我们构建的称为NanoArrayer的仪器的消耗性组件（在NIH R44 EB00316的支持下）。它们还将被配置为与商用原子力显微镜兼容。在拟议的第二阶段计划中，我们将专注于增加在单个打印周期中创建的单个斑点的数量，同时保持高度的可重复性和最小的样品交叉污染。我们将优化线性阵列（1-D） spt，其中包含10个单独加载的悬臂，然后在第二维扩展，最终在平面二维打印头中同时打印多达100个纳米阵列点，每个点能够包含一个独特的生物分子物种。重点将放在控制样品溢出（交叉污染），斑点大小，印刷报告性和spt表面对齐。我们还将开始探索在局部磁场悬臂控制、替代（非微悬臂）SPT设计和软聚合物印刷基板等领域的前瞻性发展，这些基板可用于微流体和通过接触印刷进行纳米射线克隆。在第二阶段计划结束时，我们将为各种超微和纳米阵列应用提供基于stp的解决方案。我们的商业化计划将在整个二期开发项目中继续进行，并在研发过程中引入改进的spt。