Bioengineering Nanotechnology Initiative

描述(申请人提供)：在该项目的第一阶段可行性研究中 研究计划我们成功地构建并测试了一个原型 纳米阵列。此第二阶段提案的目标是构建和测试 第二代NanoArrayerTM这个装置将能够定位 固体表面上的分子具有纳米尺度的区域大小和分辨率。 NanoArrayer II将包括一个复杂的力反馈机制，用于 沉积工具，样本源定位系统，以允许轻便 预构型分子中多种分子物种的沉积 微阵列，以及更强大和更美化的软件，包括直观的 图形用户界面(GUI)。几种类型的纳米阵列分析将是 开发，专注于免疫诊断应用。纳米阵列设备 在该计划第二阶段开发的纳米阵列测试将提供 与现有分子评价方法相比具有显著优势 互动，包括通过以下方式大幅减少材料使用量 小型化、实时数据采集和无标记化验 环境。该技术具有广泛的商业适用性，具有关键 目标领域包括免疫诊断、蛋白质-蛋白质相互作用 (蛋白质组学)和药物发现。一项重大的商业化努力将 在此技术的第二阶段开始，并在第三阶段升级 制定和实施计划。 建议的商业应用： 商业应用包括筛选大量的蛋白质-蛋白质相互作用 用于蛋白质组分析、多重免疫诊断和快速、无标记的候选药物 在大分子文库中的评价。

DESCRIPTION (provided by applicant): In the Phase I feasibility study for this research program we successfully constructed and tested a prototype NanoArrayer. The goal of this Phase II proposal is to construct and test a second generation NanoArrayerTM. This device will be capable of positioning molecules on a solid surface with nanometer scale domain sizes and resolution. The NanoArrayer II will include a sophisticated force feedback mechanism for the deposition tool, a sample source positioning system to allow facile deposition of multiple molecular species from pre-configured molecular microarrays, and more robust and embellished software, including an intuitive graphical user interface (GUI). Several types of NanoArray assays will be developed, focusing on immunodiagnostic applications. The NanoArrayer device and the NanoArray tests developed in Phase II of this program will provide significant advantages over existing methods for evaluating molecular interactions, including vast reductions in materials usage through miniaturization, real time data acquisition, and a label-free assay environment. This technology has broad commercial applicability, with key target areas including immunodiagnostics, protein-protein interactions (proteomics), and drug discovery. A significant commercialization effort will commence during phase II and escalate in Phase III of this technology development and implementation program. PROPOSED COMMERCIAL APPLICATION: Commercial applications include screening for large number of protein-protein interactions for proteomic analysis, multiplexed immunodiagnostics, and rapid, label free drug candidate evaluation in large molecular libraries.