Bioengineering Nanotechnology Initiative

生物工程纳米技术倡议

• 批准号: 6337070
• 负责人: Eric R HENDERSON
• 金额: $ 62.84万
• 依托单位: BIOFORCE NANOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6337070
• 关键词: atomic force microscopy; bioengineering /biomedical engineering; biomedical equipment development; biotechnology; intermolecular interaction; microarray technology; miniature biomedical equipment; nanotechnology; technology /technique development

DESCRIPTION (provided by applicant): In the Phase I feasibility study for this research program we successfully constructed and tested a prototype NanoArrayer. The goal of this Phase II proposal is to construct and test a second generation NanoArrayerTM. This device will be capable of positioning molecules on a solid surface with nanometer scale domain sizes and resolution. The NanoArrayer II will include a sophisticated force feedback mechanism for the deposition tool, a sample source positioning system to allow facile deposition of multiple molecular species from pre-configured molecular microarrays, and more robust and embellished software, including an intuitive graphical user interface (GUI). Several types of NanoArray assays will be developed, focusing on immunodiagnostic applications. The NanoArrayer device and the NanoArray tests developed in Phase II of this program will provide significant advantages over existing methods for evaluating molecular interactions, including vast reductions in materials usage through miniaturization, real time data acquisition, and a label-free assay environment. This technology has broad commercial applicability, with key target areas including immunodiagnostics, protein-protein interactions (proteomics), and drug discovery. A significant commercialization effort will commence during phase II and escalate in Phase III of this technology development and implementation program. PROPOSED COMMERCIAL APPLICATION: Commercial applications include screening for large number of protein-protein interactions for proteomic analysis, multiplexed immunodiagnostics, and rapid, label free drug candidate evaluation in large molecular libraries.

描述（由申请人提供）：在第一阶段可行性研究中 我们成功构建并测试了原型的研究计划 纳米阵列仪。第二阶段提案的目标是构建和测试 第二代 NanoArrayerTM。该设备将能够定位 固体表面上的分子具有纳米级的域尺寸和分辨率。 NanoArrayer II 将包括一个复杂的力反馈机制 沉积工具，一个样品源定位系统，可以方便地 从预先配置的分子中沉积多种分子种类 微阵列，以及更强大和更完善的软件，包括直观的 图形用户界面（GUI）。几种类型的纳米阵列检测将被 开发，专注于免疫诊断应用。 NanoArrayer 设备 该计划第二阶段开发的纳米阵列测试将提供 与现有分子评估方法相比具有显着优势 相互作用，包括通过以下方式大幅减少材料使用 小型化、实时数据采集和无标记测定 环境。该技术具有广泛的商业适用性，关键 目标领域包括免疫诊断、蛋白质-蛋白质相互作用 （蛋白质组学）和药物发现。重大的商业化努力将 在该技术的第二阶段开始并在第三阶段升级 制定和实施计划。 拟议的商业应用： 商业应用包括筛选大量蛋白质-蛋白质相互作用 用于蛋白质组分析、多重免疫诊断和快速、无标记候选药物 大型分子库中的评估。