Bioengineering Nanotechnology Initiative

描述（由申请人提供）：在第一阶段可行性研究中， 我们成功地构建并测试了一个原型 纳米阵列第二阶段提案的目标是构建和测试一个 第二代NanoArrayerTM。这个装置将能够定位 分子在固体表面上的纳米尺度域尺寸和分辨率。 NanoArrayer II将包括一个复杂的力反馈机制， 沉积工具，样品源定位系统，以允许容易地 从预配置的分子中沉积多个分子种类 微阵列，以及更强大和修饰的软件，包括直观的 图形用户界面（GUI）。几种类型的NanoArray检测将在 开发，专注于免疫诊断应用。NanoArrayer设备 该项目第二阶段开发的纳米阵列测试将提供 与现有的用于评估分子生物学特性的方法相比， 相互作用，包括大量减少材料的使用， 微型化、真实的时间数据采集和无标记分析 环境该技术具有广泛的商业适用性， 目标领域包括免疫诊断学、蛋白质-蛋白质相互作用 （蛋白质组学）和药物发现。一项重大的商业化努力将 在第二阶段开始，在第三阶段逐步升级 发展和实施方案。 拟定商业应用： 商业应用包括筛选大量的蛋白质-蛋白质相互作用 用于蛋白质组学分析、多重免疫诊断和快速、无标记的候选药物 在大型分子文库中进行评价。

DESCRIPTION (provided by applicant): In the Phase I feasibility study for this research program we successfully constructed and tested a prototype NanoArrayer. The goal of this Phase II proposal is to construct and test a second generation NanoArrayerTM. This device will be capable of positioning molecules on a solid surface with nanometer scale domain sizes and resolution. The NanoArrayer II will include a sophisticated force feedback mechanism for the deposition tool, a sample source positioning system to allow facile deposition of multiple molecular species from pre-configured molecular microarrays, and more robust and embellished software, including an intuitive graphical user interface (GUI). Several types of NanoArray assays will be developed, focusing on immunodiagnostic applications. The NanoArrayer device and the NanoArray tests developed in Phase II of this program will provide significant advantages over existing methods for evaluating molecular interactions, including vast reductions in materials usage through miniaturization, real time data acquisition, and a label-free assay environment. This technology has broad commercial applicability, with key target areas including immunodiagnostics, protein-protein interactions (proteomics), and drug discovery. A significant commercialization effort will commence during phase II and escalate in Phase III of this technology development and implementation program. PROPOSED COMMERCIAL APPLICATION: Commercial applications include screening for large number of protein-protein interactions for proteomic analysis, multiplexed immunodiagnostics, and rapid, label free drug candidate evaluation in large molecular libraries.