BioMarker Analysis from Single Cells using Nanobiosensor

使用纳米生物传感器对单细胞进行生物标志物分析

• 批准号: 7050861
• 负责人: Eric R HENDERSON
• 金额: $ 20.43万
• 依托单位: BIOFORCE NANOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7050861
• 关键词: actins; antigen antibody reaction; bioengineering /biomedical engineering; biomarker; biomedical equipment development; biosensor device; cell sorting; cytology; enzyme linked immunosorbent assay; interleukin 2; intracellular; microarray technology; molecular probes; nanotechnology; photomicrography; printing; proteomics; secretory protein; serine threonine protein kinase; tumor necrosis factor alpha

DESCRIPTION (provided by applicant): The purpose of this Phase I feasibility study to design, construct and evaluate an ultraminiaturized sensor device capable of detect ng protein biomarkers from a few cells and, ultimately, single cells. The device, termed the nanobiosensor, consists of an array of ultraminiaturized protein captur e (antibody) domains printed on a micro fabricated silicon structure. The nanobiosensor combines concepts, methods and instrumentation from nanotechnology and cel I and molecular biology to create a pathway to single-cell proteomic analysis. The nanobiosensor will be used for monitoring both secreted and intracellular protein expression in model cell ular systems. The design of the nanobiosensor allow s the test to be brought directly to the sample origin (in this case, a cell), eliminating the difficulties associated with transporting extremely small quantities of sample extracts to a remote test site. The pr opposed research is enabled by "NanoArrayer" technology developed by the applicant institution for the construction of biochips w itch ultramicro (1-20 mm) to submicron (250 nm) spatial addresses (corresponding to 1/1000th - 1/10,000th of the surface area required for conventional microar rays tests) and requiring only extremely small sample volumes (sub-microliter to picoliter). The nanobiosensor will permit analysis of protein profiles from samples that previously could only be analyzed via PCR amplification of nucleic acids. The proposed nanobiosensor opens the door to fast and infor mative analysis of minute protein samples such as those found in forensics s ituations, neonates and tissue subpopulations (e.g., laser cell microdissected biopsy samples). This level of miniaturization translates to minimally invasive yet data-rich molecular diagnostics for early detection of cancer and other diseases with a significant reduction in patient discomfort.

描述（由申请人提供）：本I期可行性研究的目的是设计、构建和评估一种能够从少数细胞和最终单个细胞中检测ng蛋白生物标志物的超小型化传感器设备。该装置被称为纳米生物传感器，由一组印刷在微制造硅结构上的超小型化蛋白质捕获e（抗体）结构域组成。纳米生物传感器结合了纳米技术、细胞I和分子生物学的概念、方法和仪器，创造了单细胞蛋白质组学分析的途径。该纳米生物传感器将用于监测模型细胞系统中分泌和细胞内蛋白的表达。纳米生物传感器的设计允许将测试直接带到样品原点（在这种情况下，是一个细胞），消除了将极少量的样品提取物运送到远程测试地点的困难。所反对的研究是由申请机构开发的“纳米阵列”技术实现的，该技术用于构建生物芯片，其空间地址从超微（1-20毫米）到亚微米（250纳米）（相当于传统微射线测试所需表面积的1/1000 - 1/10,000），并且只需要极小的样品体积（亚微升到皮升）。纳米生物传感器将允许分析样品的蛋白质谱，以前只能通过核酸扩增的PCR分析。提议的纳米生物传感器打开了快速和信息分析微小蛋白质样品的大门，例如在法医情况下，新生儿和组织亚群（例如，激光细胞微解剖活检样品）中发现的样品。这种微型化水平转化为微创但数据丰富的分子诊断，可用于癌症和其他疾病的早期检测，显著减少患者的不适。