Microfabricated Deposition Tools for Creating NanoArrays

用于创建纳米阵列的微加工沉积工具

• 批准号: 6697424
• 负责人: Eric R HENDERSON
• 金额: $ 20万
• 依托单位: BIOFORCE NANOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6697424
• 关键词: biomaterial development /preparation; biotechnology; method development; miniature biomedical equipment; nanotechnology

DESCRIPTION (provided by applicant): The goal of this proposal is to design and test novel microcantilever devices for the purpose of improving deposition of biomaterials in ultraminiaturized arrays. BioForce NanoSciences, Inc. (http://www.bioforcelab.com) is an industry leader in the development of next-generation biomolecular screening arrays, or NanoArrays, with patented technology permitting the construction of biochips with sub-micron spatial addresses. These nano-scale arrays allow a thousand or more molecular tests to be carried out in the same surface space occupied by a single state-of-the-art microarray spot. This format offers dramatic savings in reagent cost that accompanies ultra-miniaturization, and more importantly, it provides a realistic and sought-after platform for performance of array-based analyses in applications involving extremely small quantities of sample material where uses of current microarray formats are not feasible. These applications include single-cell protein profiling, prenatal diagnostics, forensic testing, high-throughput drug screening, and minimally invasive clinical diagnostic testing. Commercialization of this technology requires highly reproducible molecular deposition that is not feasible with current deposition tools. This research project will advance this technology through the following specific aims: 1. Design and construct front and back loading quill- and aperture-type single, and multiplexed deposition tools; and 2. compare iterative deposition-wash cycle with pre-load, continuous flow deposition methods using the devices designed in aim 1.

描述（由申请人提供）：本提案的目标是设计和测试新型微悬臂梁装置，以改善超小型阵列中生物材料的沉积。BioForce NanoSciences，Inc. (http：www.bioforcelab.com）是开发下一代生物分子筛选阵列或NanoArrays的行业领导者，其专利技术允许构建具有亚微米空间地址的生物芯片。这些纳米级阵列允许在由单个最先进的微阵列点占据的相同表面空间中进行一千个或更多的分子测试。这种格式提供了极大的节省试剂成本，伴随着超小型化，更重要的是，它提供了一个现实的和抢手的平台，用于在涉及极少量的样品材料的应用中进行基于阵列的分析，其中使用当前的微阵列格式是不可行的。这些应用包括 单细胞蛋白质谱分析、产前诊断、法医检测、高通量药物筛选和微创临床诊断检测。该技术的商业化需要高度可再现的分子沉积，这在当前的沉积工具中是不可行的。本研究项目将通过以下具体目标推进这项技术：1。设计和构造前、后加载套筒式和孔径式单个和多路沉积工具;以及2.使用目标1中设计的装置，将迭代沉积-洗涤循环与预加载、连续流沉积方法进行比较。