Microfabricated Deposition Tools for Creating NanoArrays

用于创建纳米阵列的微加工沉积工具

• 批准号: 6697424
• 负责人: Eric R HENDERSON
• 金额: $ 20万
• 依托单位: BIOFORCE NANOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6697424
• 关键词: biomaterial development /preparation; biotechnology; method development; miniature biomedical equipment; nanotechnology

DESCRIPTION (provided by applicant): The goal of this proposal is to design and test novel microcantilever devices for the purpose of improving deposition of biomaterials in ultraminiaturized arrays. BioForce NanoSciences, Inc. (http://www.bioforcelab.com) is an industry leader in the development of next-generation biomolecular screening arrays, or NanoArrays, with patented technology permitting the construction of biochips with sub-micron spatial addresses. These nano-scale arrays allow a thousand or more molecular tests to be carried out in the same surface space occupied by a single state-of-the-art microarray spot. This format offers dramatic savings in reagent cost that accompanies ultra-miniaturization, and more importantly, it provides a realistic and sought-after platform for performance of array-based analyses in applications involving extremely small quantities of sample material where uses of current microarray formats are not feasible. These applications include single-cell protein profiling, prenatal diagnostics, forensic testing, high-throughput drug screening, and minimally invasive clinical diagnostic testing. Commercialization of this technology requires highly reproducible molecular deposition that is not feasible with current deposition tools. This research project will advance this technology through the following specific aims: 1. Design and construct front and back loading quill- and aperture-type single, and multiplexed deposition tools; and 2. compare iterative deposition-wash cycle with pre-load, continuous flow deposition methods using the devices designed in aim 1.

描述（由申请人提供）：该提案的目的是设计和测试新型的微型管理器设备，以改善超显影阵列中生物材料的沉积。 BioForce Nanosciences，Inc。（http://www.bioforcelab.com）是开发下一代生物分子筛查阵列或纳米阵列的行业领导者，并具有专利的技术，允许使用次级微米的空间地址来构建生物芯片。这些纳米尺度阵列允许在单个最先进的微阵列占据的同一表面空间中进行一千个或更多的分子测试。这种格式为超微小化的试剂成本提供了巨大的节省，更重要的是，它为在涉及极少量样品材料的应用中提供基于阵列的分析提供了一个现实且引人注目的平台，而当前微阵列格式的使用不可行。这些应用包括单细胞蛋白分析，产前诊断，法医测试，高通量药物筛查以及微创临床诊断测试。该技术的商业化需要高度可重现的分子沉积，而当前沉积工具不可行。该研究项目将通过以下特定目的推进这项技术：1。设计和构建前后负载鹅毛笔和孔径单型单件和多重沉积工具；和2。使用AIM 1中设计的设备将迭代沉积洗涤周期与预载的连续流沉积方法进行比较。