Engineering Development of Laser Capture Microdissection

激光捕获显微切割的工程开发

• 批准号: 6112720
• 负责人: SETH GOLDSTEIN
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6112720
• 关键词: bioengineering /biomedical engineering; dissection; histopathology; lasers; method development; molecular film; molecular pathology; thermography

Laser Capture Microdissection is a technique developed at NIH to capture minute portions of tissue from histo-pathology slides for subsequent molecular analysis. A thermoplastic film in contact with a tissue specimen is selectively activated by a laser beam under microscopic control to bond with the desired tissue. Removal of the film separates the adhered tissue from that remaining on the slide, making it available for analysis. The technique enables the collection and processing of extremely homogeneous samples in a routine manner that does not require great manual dexterity. Over the past several years a number of refinements of the technique have been developed. A cylindrical configuration employing a "line" contact with the tissue has been perfected which minimizes contamination, and is capable of removing tissue fragments as small as 3 microns in diameter. Several systems utilizing a continuous tape of thermoplastic are under development. A thermal model has been developed to describe the process by which the laser energy is absorbed and diffused in the polymer. This enables the prediction of tissue and film temperature transients as a function of the system parameters.

激光捕获显微切割技术是一种 在NIH开发的捕捉组织的微小部分， 组织病理学切片用于随后的分子分析。一 与组织样本接触的热塑性膜选择性地 在显微镜控制下被激光束激活， 想要的组织移除薄膜可分离粘附的组织 从幻灯片上剩下的，使其可用于分析。 该技术能够收集和处理极其复杂的 以常规方式采集均匀样品， 非常灵巧的手在过去几年中， 已经开发了该技术的改进。圆柱形 采用与组织“线”接触的构造已经被 完善，最大限度地减少污染，并能够 移除直径小至3微米的组织碎片。 使用连续热塑性带的几种系统是 正在开发中。已经开发了一个热模型， 描述激光能量被吸收的过程， 在聚合物中扩散。这使得能够预测组织和 作为系统参数的函数的膜温度瞬变。