ENGINEERING DEVELOPMENT OF LASER CAPTURE MICRODISSECTION

激光捕获显微切割是NIH开发的一种技术，用于从组织病理学切片中捕获微小的组织部分，用于随后的分子分析。在显微控制下，通过激光束选择性地激活与组织样本接触的热塑性膜，以与所需组织结合。移除膜将粘附的组织与载玻片上剩余的组织分离，使其可用于分析。该技术能够以常规方式收集和处理非常均匀的样品，不需要很大的手动灵活性。在过去的几年中，已经开发了一些改进的技术。我们已经完善了一个圆柱形配置，它采用与组织的线接触，以尽量减少污染。这能够去除直径小至3微米的组织碎片。我们将其进一步开发为非接触式系统，以优化单细胞转移系统。我们还在开发一种非接触系统，该系统利用热塑性塑料的连续带，该连续带通过约5微米的间隙与组织分离。胶带和组织之间的接触仅发生在激光脉冲的直接位置。我们希望探索是否有性能和经济优势，使用这种替代基板。我们正在改进我们的热模型，该模型描述了激光能量在聚合物中吸收和扩散的过程，以进一步预测组织和薄膜温度瞬变随系统参数的变化。- 显微切割，激光捕获显微切割，组织显微切割，激光

Laser Capture Microdissection is a technique developed at NIH to capture minute portions of tissue from histo-pathology slides for subsequent molecular analysis. A thermoplastic film in contact with a tissue specimen is selectively activated by a laser beam under microscopic control to bond with the desired tissue. Removal of the film separates the adhered tissue from that remaining on the slide, making it available for analysis. The technique enables the collection and processing of extremely homogeneous samples in a routine manner that does not require great manual dexterity. Over the past several years a number of refinements of the technique have been developed. We have perfected a cylindrical configuration which employs a line contact with the tissue to minimize contamination. This is capable of removing tissue fragments as small as 3 microns in diameter. We have further developed this into a non-contact system to optimize the system for single cell transfers. We are also developing a non-contact system utilizing a continuous tape of thermoplastic which is separated from the tissue by a gap of approximatly 5 microns. Contact between the tape and the tissue will only occur at the immediate location of the laser pulse. We hope to explore whether there are performance and economic advantages to using this alternative substrate. We are refining our thermal model which describes the process by which the laser energy is absorbed and diffused in the polymer in order to further predict tissue and film temperature transients as a function of the system parameters. - microdissection, laser- capture- microdissection, tissue- microdissection, lasers