Engineering Development of Laser Capture Microdissection

激光捕获显微切割的工程开发

• 批准号: 6432971
• 负责人: SETH GOLDSTEIN
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6432971
• 关键词: bioengineering /biomedical engineering; dissection; histopathology; lasers; method development; molecular film; molecular pathology; thermography

Laser Capture Microdissection is a technique developed at NIH to capture minute portions of tissue from histo-pathology slides for subsequent molecular analysis. A thermoplastic film in contact with a tissue specimen is selectively activated by a laser beam under microscopic control to bond with the desired tissue. Removal of the film separates the adhered tissue from that remaining on the slide, making it available for analysis. The technique enables the collection and processing of extremely homogeneous samples in a routine manner that does not require great manual dexterity. Over the past several years a number of refinements of the technique have been developed. We have perfected a cylindrical configuration which employs a "line" contact with the tissue to minimize contamination. This is capable of removing tissue fragments as small as 3 microns in diameter. We have further developed this into a non-contact system to optimize the system for single cell transfers. We have also developed a non-contact system utilizing a continuous tape of thermoplastic which is separated from the tissue by a gap of approximately 5 microns. Contact between the tape and the tissue will only occur at the immediate location of the laser pulse. We are exploring whether there are performance and economic advantages to using this alternative substrate.

激光捕获显微切割是 NIH 开发的一项技术，用于从组织病理学载玻片中捕获组织的微小部分，以进行后续的分子分析。 与组织样本接触的热塑性薄膜在微观控制下被激光束选择性激活，以与所需的组织结合。去除薄膜将粘附的组织与载玻片上剩余的组织分开，使其可用于分析。该技术能够以常规方式收集和处理极其均匀的样品，不需要很高的手工灵活性。 在过去的几年里，该技术得到了许多改进。 我们完善了圆柱形配置，采用与组织的“线”接触来最大限度地减少污染。 它能够去除直径小至 3 微米的组织碎片。我们将其进一步开发为非接触式系统，以优化单细胞转移系统。我们还开发了一种非接触式系统，利用连续的热塑性塑料带，该带与组织之间的间隙约为 5 微米。 胶带和组织之间的接触只会发生在激光脉冲的直接位置。 我们正在探索使用这种替代基材是否具有性能和经济优势。