课题基金 / 基金详情

QUANTITATION OF EPR SPECTRA BY SPECTRAL SIMULATION

QUANTITATION OF EPR SPECTRA BY SPECTRAL SIMULATION
通过光谱模拟对 EPR 光谱进行定量
批准号:
6118813
负责人:
NEIL HOGG
金额:
$0.21万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-03-01 至 2000-02-29

项目摘要

项目成果

NEIL HOGG的其他基金

相似基金

相关文献

中文摘要
翻译
电子顺磁共振(EPR)的自旋捕获已经被 广泛用于检测人体内瞬时自由基的形成 生物和化学系统。这项技术使用了一个自旋陷阱, 与自由基反应形成持久的自旋加合物,这 可通过电子顺磁共振直接检测。在许多情况下,激进分子 识别可以作为特定的自旋加合物来完成 自由基具有独特的光谱特征。自旋俘获通常 用于定性的方式,因为自旋加合物通常也 浓度低和/或太不稳定,不能准确 量化。良好的信噪比对于 由于EPR谱必须进行两次积分,因此需要进行精确的量化 以求出样品中自由基的浓度。 我们已经开发出一种方法,信号较差的光谱可以 噪音是可以量化的。该方法使用光谱模拟来 生成Ori Gina L谱的无噪声表示,它 然后可以通过积分进行定量分析。浓缩度 可以测定复杂混合物中单个自旋加合物的含量。在……里面 此外,还可以分析自由基产生的动力学,作为信号 不需要采用平均化。这种方法已经被用来 黄嘌呤/黄嘌呤生成超氧化物动力学的测定 氧化酶和一氧化氮合酶。
英文摘要
Electron paramagnetic resonance (EPR) spin-trapping has been extensively used to detect the formation of transient free radicals in biological and chemical systems. The technique uses a spin-trap that reacts with the free radical to form a persistent spin-adduct, which can be detected directly by EPR. In many cases, radical identification can be accomplished as the spin-adduct of a particular radical has unique spectral features. Spin-trapping has generally been used in a qualitative manner as the spin-adducts are usually too low in concentration and/or too unstable to allow accurate quantification. A good signal to noise ratio is essential for accurate quantification as the EPR spectrum has to be integrated twice in order to derive the concentration of free radical in the sample. We have developed a methodology whereby spectra with poor signal to noise can be quantified. This method uses a spectral simulation to generate a noise free representation of the ori gina l spectrum, which can then be quantitatively analyzed by integration. The concentration of individual spin adducts in complex mixtures can be determined. In addition the kinetics of radical production can be analyzed, as signal averaging need not be employed. This approach has been used to determine the kinetics of superoxide formation from xanthine/xanthine oxidase and nitric oxide synthase.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
2012 Oxygen Radicals Gordon Research Conference
  • 批准号:
    8255109
  • 项目类别:
  • 资助金额:
    $2.25万
  • 财政年份:
    2012
  • 负责人:
    NEIL HOGG
  • 依托单位:
Summer Research Experiences for Undergraduates
  • 批准号:
    6561443
  • 项目类别:
  • 资助金额:
    $5.95万
  • 财政年份:
    2003
  • 负责人:
    NEIL HOGG
  • 依托单位:
Summer Research Experiences for Undergraduates
  • 批准号:
    6737558
  • 项目类别:
  • 资助金额:
    $6.11万
  • 财政年份:
    2003
  • 负责人:
    NEIL HOGG
  • 依托单位:
Summer Research Experiences for Undergraduates
  • 批准号:
    7057787
  • 项目类别:
  • 资助金额:
    $6.45万
  • 财政年份:
    2003
  • 负责人:
    NEIL HOGG
  • 依托单位:
海外基金