QUANTITATION OF EPR SPECTRA BY SPECTRAL SIMULATION

通过光谱模拟对 EPR 光谱进行定量

• 批准号: 6279833
• 负责人: NEIL HOGG
• 金额: $ 1.13万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6279833
• 关键词: bioengineering /biomedical engineering; biomedical resource; computers; technology /technique

Electron paramagnetic resonance (EPR) spin-trapping has been extensively used to detect the formation of transient free radicals in biological and chemical systems. The technique uses a spin-trap that reacts with the free radical to form a persistent spin-adduct, which can be detected directly by EPR. In many cases, radical identification can be accomplished as the spin-adduct of a particular radical has unique spectral features. Spin-trapping has generally been used in a qualitative manner as the spin-adducts are usually too low in concentration and/or too unstable to allow accurate quantification. A good signal to noise ratio is essential for accurate quantification as the EPR spectrum has to be integrated twice in order to derive the concentration of free radical in the sample. We have developed a methodology whereby spectra with poor signal to noise can be quantified. This method uses a spectral simulation to generate a noise free representation of the original spectrum, which can then be quantitatively analyzed by integration. The concentration of individual spin adducts in complex mixtures can be determined. In addition the kinetics of radical production can be analyzed, as signal averaging need not be employed. This approach has been used to determine the kinetics of superoxide formation from xanthine/xanthine oxidase and nitric oxide synthase.

电子顺磁共振（EPR）自旋捕获已被研究