TRAINING IN TECHNOLOGY FOR IMAGE ANALYSIS & SPECIMEN PREPARATION

今年我们的设施接待了三位游客，他们每人花了 与我们一起学习各种标本制备方法， 计算机辅助图像分析。 两位访客卡尔医生 Ekwall和Trisha Davis参加了小型研讨会， 教学标本制备，免疫定位，断层扫描和系列 重建 这些小型研讨会导致了官方 Ekwall博士和Davis博士的合作。 此外，我们长期以来 合作者，玛丽波特博士参观了实验室，学习步骤 用于衣原体轴丝动力蛋白的图像分析。 在波特博士访问期间，她还会见了大卫马斯特罗纳德， Eileen O 'Toole获取设置图像所需的程序 她在明尼苏达州的实验室里进行了分析。 巨石 三维精细结构实验室主页 (http：bio3d.colorado.edu）也已更新， web安装选项下载我们实验室开发的软件。 的 IMOD建模软件仍然是一个有用的程序，为我们的 实验室和世界各地的几个实验室。 几 大卫已经对IMOD计划进行了改进 Mastronarde进一步提高其实用性

This year our facility hosted three visitors, each of whom spent one week with us to learn various methods of specimen preparation and computer-assisted image analysis. Two of the visitors, Drs. Karl Ekwall and Trisha Davis, participated in mini-workshops designed to teach specimen preparation, immunolocalization, tomography, and serial reconstruction. These mini-workshops have led to official collaborations with Drs. Ekwall and Davis. In addition, our long-time collaborator, Dr. Mary Porter visited the lab to learn the steps required for image analysis of axonemal dynein in Chlamydomonas. During Dr. Porter~s visit she also met with David Mastronarde and Eileen O'Toole to obtain the programs needed to set up the image analysis protocols in her laboratory in Minnesota. The Boulder Laboratory for 3-D Fine Structure home page (http://bio3d.colorado.edu) has also been updated to contain a web-install option to download the software developed in our lab. The IMOD modeling software continues to be a useful program for our laboratory and to several laboratories around the world. Several improvements to the IMOD program have been implemented by David Mastronarde further to improve its usefulness