TRAINING IN TECHNOLOGY FOR IMAGE ANALYSIS & SPECIMEN PREPARATION

今年我们的设施接待了三位参观者，每个人都花了 与我们一起学习各种标本制备方法和 计算机辅助图像分析。两位来访者，卡尔博士 埃克沃尔和特丽莎·戴维斯参加了旨在 讲授标本制备、免疫定位、断层扫描和系列课程 重建。这些迷你研讨会导致了官方 与埃克沃尔博士和戴维斯博士的合作。另外，我们长期以来 合作者玛丽·波特博士参观了实验室，了解了这些步骤 衣藻中轴内动蛋白的图像分析所必需的。 在波特~S博士访问期间，她还会见了大卫·马斯特罗纳德和 Eileen O‘Toole获取设置图像所需的程序 她在明尼苏达州的实验室里的分析方案。巨石 三维精细结构实验室主页 (http://bio3d.colorado.edu)也已更新，包含一个 Web安装选项，以下载我们实验室开发的软件。这个 IMOD建模软件继续是我们的 实验室和世界各地的几个实验室。几个 对IMOD方案的改进已由David实施 Mastronarde进一步提高其有用性

This year our facility hosted three visitors, each of whom spent one week with us to learn various methods of specimen preparation and computer-assisted image analysis. Two of the visitors, Drs. Karl Ekwall and Trisha Davis, participated in mini-workshops designed to teach specimen preparation, immunolocalization, tomography, and serial reconstruction. These mini-workshops have led to official collaborations with Drs. Ekwall and Davis. In addition, our long-time collaborator, Dr. Mary Porter visited the lab to learn the steps required for image analysis of axonemal dynein in Chlamydomonas. During Dr. Porter~s visit she also met with David Mastronarde and Eileen O'Toole to obtain the programs needed to set up the image analysis protocols in her laboratory in Minnesota. The Boulder Laboratory for 3-D Fine Structure home page (http://bio3d.colorado.edu) has also been updated to contain a web-install option to download the software developed in our lab. The IMOD modeling software continues to be a useful program for our laboratory and to several laboratories around the world. Several improvements to the IMOD program have been implemented by David Mastronarde further to improve its usefulness