TRAINING IN TECHNOLOGY FOR IMAGE ANALYSIS & SPECIMEN PREPARATION

今年，Mary Morphew举办了两次为期两天的EM研讨会 快速冷冻法和冷冻置换法制备样品 澳大利亚墨尔本大学。她准备了一本手册 在研讨会上分发描述标本的技术 制备和抗原定位。此外，我们的实验室还主办了 四名参观者每人花了一周的时间学习标本准备 和图像分析。这些访问被设置为小型研讨会，在那里 参观者将与实验室的每一名成员共度时光。一个详细的 介绍轴突图像分析所涉及的步骤的手册 衣藻中的动力蛋白也已经组装完毕。巨石 3D精细结构实验室现在有一个广泛的主页 万维网(http://bio3d@colorado.edu/).这个主页， 由Jim Kremer创建，已经成为关于 我们的实验室，以及所有图像软件和 免费提供的用户指南。大约1个 已有数百人从该网站下载了IMOD软件。 最近，添加了远程显微镜程序webScope 到网页，我们希望我们可以发展一个新的用户基础与 这个节目是通过互联网进行的。

This year, Mary Morphew gave two, 2-day workshops on EM preparation of specimens by fast freezing and freeze substitution at the University of Melbourne, Australia. She prepared a manual to be distributed at the workshop describing techniques for specimen preparation and antigen localization. In addition, our lab hosted four visitors who each spent one week learning specimen preparation and image analysis. These visits were set up as mini-workshops where the visitor would spend time with each member of the Lab. A detailed manual describing the steps involved for image analysys of axonemal dynein in Chlamydomonas has also been assembled. The Boulder Laboratory for 3-D Fine Structure now has an extensive home page on the World Wide Web (http://bio3d@colorado.edu/). This home page, created by Jim Kremer, has become a useful source of information about our laboratory, as well as a source for all of the image software and user's guides that are available free of charge. Approximately one hundred people have downloaded the IMOD software from this web site. More recently the remote microscopy program, webScope, has been added to the web page, and we hope that we can develop a new user base with this program via the Internet.