TRAINING IN TECHNOLOGY FOR IMAGE ANALYSIS & SPECIMEN PREPARATION

今年，玛丽墨菲给了两个，为期两天的研讨会上EM 用快速冷冻和冷冻置换法制备试样. 澳大利亚墨尔本大学。 她准备了一本手册， 在讲习班上分发，介绍了标本制作技术， 制备和抗原定位。 此外，我们的实验室还举办了 四名参观者每人花了一周时间学习标本制备 和图像分析。 这些访问被设置为小型研讨会， 参观者将与实验室的每一个成员呆在一起。详细 轴丝图像分析步骤手册 衣原体中的动力蛋白也被组装起来。 巨石 三维精细结构实验室现在有一个广泛的主页， 万维网（http：//bio3d@colorado.edu/）。这个主页， 由Jim Kremer创建，已成为一个有用的信息来源， 我们的实验室，以及所有图像软件的来源， 免费提供的用户指南。 大约一 已经有数百人从这个网站下载了IMOD软件。 最近又增加了远程显微镜程序webScope 到网页，我们希望我们可以开发一个新的用户群， 通过互联网访问此程序。

This year, Mary Morphew gave two, 2-day workshops on EM preparation of specimens by fast freezing and freeze substitution at the University of Melbourne, Australia. She prepared a manual to be distributed at the workshop describing techniques for specimen preparation and antigen localization. In addition, our lab hosted four visitors who each spent one week learning specimen preparation and image analysis. These visits were set up as mini-workshops where the visitor would spend time with each member of the Lab. A detailed manual describing the steps involved for image analysys of axonemal dynein in Chlamydomonas has also been assembled. The Boulder Laboratory for 3-D Fine Structure now has an extensive home page on the World Wide Web (http://bio3d@colorado.edu/). This home page, created by Jim Kremer, has become a useful source of information about our laboratory, as well as a source for all of the image software and user's guides that are available free of charge. Approximately one hundred people have downloaded the IMOD software from this web site. More recently the remote microscopy program, webScope, has been added to the web page, and we hope that we can develop a new user base with this program via the Internet.