通过 HPLC 测量血浆甘油特异性活性以确定甘油通量-猫眼课题宝

通过 HPLC 测量血浆甘油特异性活性以确定甘油通量

批准号：

6118533

负责人：

RL L JUDD

金额：

$0.32万

依托单位：

WASHINGTON UNIVERSITY

依托单位国家：

美国

项目类别：

财政年份：

1998

资助国家：

美国

项目状态：

已结题

起止时间：

1998-08-01 至 1999-07-31

来源：

https://reporter.nih.gov/project-details/6118533

关键词：

biological products biomedical resource endocrine gland /system lipids spectrometry technology /technique

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中文摘要

翻译

以前测量血浆甘油比活性的方法

英文摘要

Previous methods for measuring plasma glycerol specific activity (SA) are sub-optimal, making the determination of glycerol kinetics in vivo with radio-tracers difficult. A new high performance liquid chromatography (HPLC) method is described which permits the accurate and specific measurement of glycerol SA. The method involves isolation of glycerol from plasma and the formation of a tribenzoyl derivative. Glycerol rate of appearance was measured in five human volunteers using both [2-3H] glycerol and [2H5] glycerol. There was close agreement between the glycerol appearance rates measured using the two approaches (1.66(0.14 vs 1.70(0.10 (mol.kg-1.min-1, respectively, P=NS). This HPLC method offers improved specificity over existing methods of measuring glycerol turnover using radio-tracers.

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